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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About SYK
SYK (Spleen-Associated Tyrosine Kinase) is a non-receptor tyrosine kinase belonging to the Syk family, closely related to ZAP-70. SYK is activated downstream of immunoreceptor tyrosine-based activation motifs (ITAMs) following engagement of B-cell receptors (BCR), Fc receptors, and integrins. Upon ITAM phosphorylation by Src-family kinases, the tandem SH2 domains of SYK engage dually phosphorylated ITAMs, triggering SYK autophosphorylation at Y352 and Y525/Y526, driving full catalytic activation. Key substrates include BLNK, PLC-gamma2, PI3K regulatory subunits, and VAV proteins, propagating NF-kB, MAPK, and calcium signaling cascades. SYK is central to B-cell development, mast cell degranulation, platelet activation, and innate immune signaling. Dysregulation drives B-cell malignancies including diffuse large B-cell lymphoma (DLBCL), chronic lymphocytic leukemia (CLL), mantle cell lymphoma, and follicular lymphoma. Elevated SYK activity also contributes to autoimmune diseases such as rheumatoid arthritis and immune thrombocytopenic purpura, making it a high-priority therapeutic target in oncology and immunology.
Why PhosphoSens for SYK?
SYK assays present several challenges: high physiological ATP concentrations in immune cells require assays tolerant of millimolar ATP, yet many luminescence-based platforms such as ADP-Glo suffer signal compression at high [ATP], underestimating potency of ATP-competitive inhibitors. HTRF and LANCE antibody-based assays introduce variability from epitope competition and require fixed endpoints that miss mechanistic detail. Radiometric formats are operationally burdensome and generate radioactive waste. For covalent SYK inhibitors, traditional endpoint formats cannot capture the full kinact/KI profile. PhosphoSens CSox-based peptide substrates enable continuous, real-time fluorescent monitoring of SYK-mediated phosphorylation at physiological ATP, generating complete progress curves. This allows precise Km(ATP) determination, accurate IC50 measurement without endpoint artifacts, and direct kinact/KI characterization of irreversible inhibitors — all in a homogeneous, antibody-free, wash-free format compatible with high-throughput screening.
Key Pathways:
B-Cell Receptor (BCR) Signaling
Fc Receptor Signaling
NF-kB Pathway
PI3K/AKT/mTOR Signaling
Approved Inhibitors:
Fostamatinib (Tavalisse, Immune Thrombocytopenia)
Talk to a Scientist
Have questions about SYK assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time SYK Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative SYK activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0794
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0794)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0794
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
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Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF SYK Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative SYK activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0653
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0653)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0653
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for SYK?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for SYK
Wild-type and mutant forms of SYK for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
SYK, Active Full Length
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-S52-10G
- Mutation: Full Length
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~102kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: SYK Assays
Can PhosphoSens SYK assays be run at physiological ATP concentrations?
Yes. PhosphoSens CSox-based assays are fully compatible with physiological ATP concentrations (1-2 mM), unlike ADP-Glo or luminescence platforms that saturate or compress signal at high [ATP]. This enables accurate determination of Km(ATP) and physiologically relevant IC50 values for ATP-competitive SYK inhibitors.
How does the PhosphoSens assay support characterization of covalent SYK inhibitors?
The continuous, real-time progress curve format of PhosphoSens allows direct measurement of time-dependent inhibition kinetics required to extract kinact and KI for covalent or irreversible SYK inhibitors. Endpoint assays such as HTRF or ADP-Glo cannot capture this time-resolved inhibition profile, leading to inaccurate potency estimates for covalent compounds.
What substrate does AssayQuant use for SYK kinase activity measurements?
AssayQuant offers optimized CSox-based peptide substrates derived from validated SYK substrate sequences, designed to be phosphorylated selectively by SYK with high sensitivity. The fluorescent signal increases continuously upon phosphorylation, enabling real-time kinetic readout without antibodies, radioactivity, or secondary detection reagents.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ SYK Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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