Real-Time Kinase and Phosphatase Assays and Services for Drug Discovery
Sensor peptide substrates built on our PhosphoSens® Technology platform form the foundation of our real-time kinase and phosphatase assays — and power our KinSight™ Services, where the same technology is used to profile compounds, quantify potency, and characterize mechanism of inhibition (MOI).
Supporting Every Phase of Preclinical Drug Discovery
From early screening through preclinical testing, AssayQuant accelerates drug discovery by turning kinetic insight into confident, data-driven decisions.
PhosphoSens Assays deliver direct, continuous measurement of kinase and phosphatase activity — converting phosphorylation events into real-time fluorescent signals for quantitative mechanistic insight. KinSight Services apply these same assays in our lab to measure compound potency, selectivity, and mechanism of inhibition across hundreds of enzyme targets. Together, these platforms enable faster, data-driven decisions from target validation through lead optimization.
Two Fit-for-Purpose Formats
Choose continuous (FI) for real-time kinetic insight or time-resolved (TRF) for screening applications.
Broad Sample Compatibility
Measure activity in recombinant enzyme systems or complex cell/tissue lysates.
Target
Validation
Confirm kinase and phosphatase targets driving key pathways.
High Throughput Screening
Screen compounds librraies using TRF readouts.
Lead
Identification
Characterize true inhibition and potency from kinetic data.
Lead
Optimization
Differentiate reversible vs. covalent inhibition and refine selectivity.
Preclinical
Testing
Evaluate activity and selectivity in cell or tissue lysates.
A Breakthrough Platform for Continuous Enzyme Activity Measurement
PhosphoSens is AssayQuant’s core technology platform that uses fluorescent sensor peptide substrates to track enzyme activity in real-time. Unlike traditional endpoint assays that only show a single measurement after stopping the reaction, PhosphoSens generates a progress curve in every well — giving you insight into reaction dynamics, inhibitor mechanism, and enzyme behaviour.
Gain a Competitive Edge with Real-Time Phosphorylation Dynamics
Characterizing targets and assessing compound potency, selectivity, and mechanism of action are critical steps in disease research and drug discovery/development, especially with the expanding focus on kinase inhibitors.
Leverage AssayQuant’s PhosphoSens® Technology to gain real-time insights into enzyme activity and inhibition. Our continuous assays simplify workflows, reduce variability, and deliver the data you need to make confident decisions.
Enzyme Activity Assay Kits, Substrates, and Recombinant Enzymes for Drug Discovery — Built on PhosphoSens Technology
AssayQuant’s patented PhosphoSens Technology powers a portfolio of over 700 unique enzyme-and-inhibitor assays for direct, real-time measurement of kinase and phosphatase activity.
Our substrates and recombinant enzymes are available in flexible configurations—whether you’re evaluating the technology in a single-plate format or running full high-throughput screens.
With validated assays for more than 500 protein kinases, 35 phosphatases, and a catalog of 1,100+ highly active enzymes, we supply everything you need to advance inhibitor discovery.
Explore our range of biochemical kinase & phosphatase activity assay kits, substrates, and supporting reagents—designed for performance, flexibility, and seamless integration in any drug-discovery laboratory.
Kinase Assays
Comprehensive kinase assay portfolio offering real-time activity and inhibition measurement with multiple product configurations.
Phosphatase Assays
Discover continuous, real-time phosphatase activity measurement with a wide selection of assays and customizable reagent options.
Lysate Assays
A direct, continuous fluorescence system for studying kinase activity and cell signaling in unfractionated lysates and tissue homogenates.
Recombinant Enzymes
Comprehensive selection of kinases and phosphatases designed for a wide range of applications, from basic research to preclinical drug discovery.
Kinase and Phosphatase Profiling Services for Potency, Selectivity, and Mechanism-of-Action Studies
AssayQuant’s KinSight Services deliver real-time kinetic insight into compound potency, selectivity, and mechanism of inhibition (MOI) using continuous enzyme activity assays built on our PhosphoSens technology platform.
Whether you’re evaluating inhibitor selectivity, confirming target engagement, or developing custom assays, KinSight provides quantitative data that reveal true enzyme kinetics — helping you make faster, more confident decisions in drug discovery.
Featured Services
Biochemical Kinase Screening and Profiling Services
Characterize compound selectivity and mechanism of action (MOA) against our panel of 400+ WT protein kinases.
Time-Dependent Inhibition Characterization
Quantify the kinetics behind inhibition with real-time data on inhibitor potency, reversibility, residence time and inactivation kinetics with confidence.
Assay Validation Reports, Detailed Protocols, Guides & Insights
Access our library of protocols, technical guides, and educational materials to support your enzyme research and drug discovery projects & browse expert-written content to help you design, troubleshoot, and optimize kinase and phosphatase assays with confidence.
