Activity-Based Biochemical Kinase Panel Screening and Profiling Services
AssayQuant's KinSight™ service helps guide improved drug discovery and development with kinetic insights into kinome-wide selectivity and mechanism of action (MOA) using our unique continuous assay format. Make better-informed decisions about therapeutic opportunities and potential off-target liabilities with high-confidence.
Go Beyond % Inhibition: Get Actionable Kinetic Insights with KinSight™ Kinase Profiling
True Kinetic Understanding:
Directly measure activity and quantify % inhibition - no endpoint assumptions - for accurate potency, selectivity, and MOA insights.
Variable ATP Concentrations for Deeper Insights:
Profile compounds at ATP Km to maximize potency data, or at 1 mM ATP for physiological relevance.
Actionable Reports, Fast:
Receive comprehensive reports with expert annotations highlighting exploitable kinetics (like TDI) – all within two weeks.
Kinase Selectivity Panels
Testing Performed
Kinase selectivity profiling using our PhosphoSens Technology generates a progress curve in everywell, providing you with actionable data you can be confident in. Selectivity screening available :
- Across a variety of predefined and customizable panels
- Offered at multiple ATP concentrations, from 1 μM to 1 mM*
- Single compound concentration or multiple doses available
- All performed in duplicate, n=2
Swiftly determine kinome-wide selectivity, gain crucial insights into mechanism of action and relative potency directly from the profiling data.
Deliverables
Detailed reports for each compound designed to guide your development decisions with annotated progress curves to call out exploitable mechanisms of actions (MOA) and % inhibition presented in:
- Numerical Format (Tabular), % inhibition for each enzyme
- Graphical Format (Waterfall Plot), % inhibition for each enzyme
- Graphical Format (Waterfall Plot), >70% inhibition
- Visual Format (Kinome Tree), >70% inhibition
Screening complete in two weeks so you get results and important functional information about the enzymology of the kinases and nature of the compound quickly!
Panel Name |
Panel Size |
ATP Options |
Target List |
|
Wild Type Protein Kinase Panel |
397 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
|
|---|---|---|---|
|
Full Protein Kinase Panel |
450 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
View Panel > |
|
Tyrosine Kinase (TK) Panel |
83 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
View Panel > |
|
Cyclin Dependent Kinase (CDK) Panel |
32 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
View Panel > |
|
Mutant Protein Kinase Panel |
53 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
View Panel > |
|
KiNode Panel |
69 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
View Panel > |
|
Custom Panel |
1-100 |
ATP Km and 1mM
now offering custom ATP concentrations*
|
Contact Us to Design > |
*Kinase selectivity profiling is conducted on a bi-monthly schedule, with assays run at the apparent ATP Km (to enable accurate potency assessment) or at 1 mM ATP (to approximate physiological conditions). Alternative ATP concentrations can be accommodated upon request.
Looking to Profile Phosphatases?
Our PhosphoSens® continuous assay technology now powers phosphatase profiling, giving you % inhibition data you can trust along with kinetic insights that endpoint assays miss against an industry leading panel of 35 phosphatases.
Stop Using Outdated Assays—Upgrade to KinSight Today
AssayQuant's KinSight™ profiling services leverage our unique PhosphoSens® Technology, a continuous assay format that directly measures substrate phosphorylation throughout the entire reaction. Instead of relying on single-point estimates, this approach generates a full progress curve in every well, yielding the actual reaction rate from dozens of data points.
Understanding the true reaction rate provides higher confidence data, free from the assumptions inherent in endpoint assays. This makes KinSight™ profiling valuable at all stages of preclinical drug discovery, helping identify compounds with poor selectivity, pinpoint optimization opportunities, or confirm off-target interactions early.
Advantages of Profiling With a Continuous Assay Format
Most kinome profiling platforms use endpoint assays—single time-point measurements that assume linear reaction kinetics. But this assumption often breaks down due to factors like lag phases, substrate depletion, enzyme instability, or time-dependent inhibition (TDI).
KinSight uses a continuous assay format to overcome these limitations. By monitoring enzyme activity in real time, we calculate actual reaction rates from multiple data points within the true linear range. This delivers higher-confidence selectivity and MOA insights, and enables direct detection of kinetic phenomena like TDI.
|
|
Detects All Types Of Kinase Inhibitors? |
Measures Kinase Activity? |
Direct Phosphorylation Detection |
Real-Time Continuous Readout |
AQT's KinSight Kinase Profiling |
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SelectScreen Profiling |
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Radiometric Assays |
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Mobility Shift Assays |
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Luminescent Assays |
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Competition Binding |
The KinSight Advantage: Real-Time Kinetic Data
Continuous (Kinetic) Activity Monitoring
Capture true inhibition kinetics for higher accuracy and deeper understanding.
Versatile ATP Concentrations
Profile at ATP Km to maximize potency, 1 mM for physiological relevance—or both for maximal MOA insights.
Informative Kinetic Profiling Report
Go beyond % inhibition with annotated kinetic data, revealing exploitable MOA insights.
Industry Leading Kinase Panel
Access 400+ quality-tested wild-type kinases, plus mutant, cascade, and custom panel options.
Correlates With Cell-Based Assays
Our technology is compatible with physiological levels of ATP, Mg2+, Mn2+, and Ca2+ and incorporates relevant activators and cofactors.
Fast Turnaround Time and Expert Guidance
Get comprehensive screening data and expert functional insights in just two weeks.
Explore an Example Data Report
Interested in exploring our full data report? Download a sample data report complete with known inhibitors and reference compounds. We tested them at variable compound concentrations with ATP concentrations at either Km or 1 mM for each of our 400+ kinases! Preview the possibilities of kinetic insights into selectivity and mechanism of action (MOA) for your drug discovery research.
Resources
AssayQuant's Kinome Profiling Services Are More Than Just an Alternative—Access A Better Approach to Kinome Profiling Today
Most biochemical kinase profiling services rely on endpoint assays, providing only a single snapshot of enzyme activity. But kinase inhibition is dynamic—why settle for incomplete data?
AssayQuant's KinSight Kinase Panel Screening and Profiling Services deliver everything you need—and more—with a next-generation approach to kinase inhibitor characterization.
With AssayQuant's KinSight profiling platform, you don’t just get data—you get kinetic insights into selectivity and mechanism of action (MOA) for your drug discovery research.
Kinome Tree Mapping Application
Visualize your kinome profiling data with customizable kinome trees.
Kinome Profiling With a Continuous Assay
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