Free Kinome Tree Generator
Turn kinase profiling data into publication-ready kinome trees. Free account, nothing to install, export as high-resolution JPG or PDF.
The AssayQuant Kinome Tree Mapping Application maps your kinase selectivity data onto the human kinome so you can see a compound's on- and off-targets at a glance. Create your free account, upload a two-column file of kinase names and percent inhibition, and color the tree with preset binary or heat map schemes, or your own custom bins.
It works with profiling data from any source, including your own lab, and is built for scientists who need a clear selectivity figure for a slide, a report or a paper.
Three Simple Steps to Create Personalized Kinome Trees:
01. Upload Your Data
To start, upload your data under Kinase & Percent Inhibition. You can manually add kinases and % inhibitions by selecting "Add New". If you have a ready-made Excel file, select "Import/Choose File" to import it and hit "Submit".
To prepare a file for upload, download our Excel template containing two columns: Column A contains the kinase names using our default naming scheme. Column B lists the percent inhibition for each kinase (without a percentage symbol).
Please note that version 1.0 of this application only recognizes a default kinase naming scheme. If you import data using an alternative naming scheme, you will receive an error message, and any incompatible kinase names will be highlighted in red.
Use our kinase naming table to find the compatible naming scheme.
Want to try the app but haven’t generated profiling data?
Download an example profiling data set run with Staurosporine (0.1 µM at 1 mM ATP) and build custom kinome trees.
Please note: if AssayQuant generated your profiling report before January 2024, it may need to be converted before uploading it to the application. For reports generated after January 2024, you will find a button to create a table formatted for automatic upload.
02. Choose Visualization Theme
You can select from pre-set binary and heat map color schemes or customize your visualization. Our pre-set binary scheme displays the kinases equal to or greater than 70% red and less than 70% green. The heat map function bins the kinases based on their % inhibition and colors each leaf, similar to a heat map.
The customization option enables you to create bins of your choice based on percent inhibition and select any color to represent that category.
03. Generate Images
To visualize your data, you only need to choose "Generate Image" at the top of the page. You can explore each kinase family by zooming in and out or resetting the image.
When you're happy with your customized kinome tree image, you can download it as a high-resolution JPG or PDF file. This will allow you to include it in your presentations and publications easily.
Need the profiling data behind the tree?
KinSight™ kinome profiling screens your compound against a panel of 560+ protein kinases using PhosphoSens® continuous assays, which read enzyme activity throughout the entire reaction instead of at a single endpoint.
- % inhibition across the panel, with a table formatted for direct upload into this app
- IC50 follow-up on the hits that matter
- Real-time progress curves that flag time-dependent inhibitors, with kinact/KI and residence time follow-up
Kinome tree FAQ
Is the kinome tree generator free?
Yes. Create a free account and use the app in your browser. There is nothing to install.
What data do I need to make a kinome tree?
A two-column file: kinase names in column A and percent inhibition in column B (numbers only, no % symbol). Download the Excel template, or start with our example staurosporine data set.
Can I use profiling data from another lab or provider?
Yes. The app accepts percent inhibition data from any source. Kinase names must match our naming scheme; check them against the kinase naming table before uploading.
Can I use the images in presentations and papers?
Yes. Download your kinome tree as a high-resolution JPG or PDF for slides, reports and publications.
What is the difference between the app and kinome profiling?
The app visualizes selectivity data you already have. KinSight kinome profiling generates that data: we test your compound against 560+ kinases with continuous PhosphoSens assays and can follow up with IC50 and time-dependent inhibition studies.
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