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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About ALK
ALK (Anaplastic Lymphoma Kinase) is a receptor tyrosine kinase belonging to the insulin receptor superfamily and the ALK/LTK subfamily. Under physiological conditions, ALK is activated by its ligands ALKAL1 and ALKAL2, triggering autophosphorylation at key tyrosine residues (Y1278, Y1282, Y1283) within the activation loop and subsequent downstream signaling. Key substrates include IRS-1, SHC, and STAT3, driving activation of RAS/MAPK, PI3K/AKT, and JAK/STAT pathways that regulate cell proliferation, survival, and differentiation. In cancer, ALK is aberrantly activated through chromosomal translocations producing fusion oncoproteins — most notably EML4-ALK in non-small cell lung cancer (NSCLC) — as well as point mutations and gene amplification seen in anaplastic large cell lymphoma (ALCL), neuroblastoma, and inflammatory myofibroblastic tumors. These oncogenic alterations make ALK one of the most clinically validated and therapeutically actionable kinase targets in oncology, with multiple generations of ALK inhibitors approved and in active clinical development.
Why PhosphoSens for ALK?
Developing ALK inhibitors presents several assay challenges: ALK fusion proteins require careful construct selection to capture relevant oncogenic activity, while resistance mutations (e.g., L1196M, G1269A) demand sensitive assays that distinguish subtle potency shifts. Endpoint assays such as ADP-Glo and HTRF require quench steps that introduce timing artifacts and miss transient inhibition kinetics critical for covalent or slow-binding inhibitors. Radiometric formats add safety and throughput burdens. AssayQuant's PhosphoSens CSox-based continuous assay monitors ALK-mediated substrate phosphorylation in real time at physiological ATP concentrations, generating full kinetic progress curves. This enables precise Km(ATP) determinations for better cellular translation, accurate IC50 measurements unaffected by endpoint timing, and direct kinact/KI measurement for covalent inhibitors — all in a homogeneous, no-antibody, mix-and-read format compatible with high-throughput screening.
Key Pathways:
RAS/MAPK signaling
PI3K/AKT/mTOR signaling
JAK/STAT3 signaling
PLCgamma signaling
Approved Inhibitors:
Crizotinib (Xalkori, ALK+ NSCLC/ROS1+ NSCLC/MET-altered NSCLC
Ceritinib (Zykadia, ALK+ NSCLC
Alectinib (Alecensa, ALK+ NSCLC
Brigatinib (Alunbrig, ALK+ NSCLC
Lorlatinib (Lorbrena, ALK+ NSCLC)
Talk to a Scientist
Have questions about ALK assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time ALK Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative ALK activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0794
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0794)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0794
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for ALK?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF ALK Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative ALK activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0653
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0653)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0653
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for ALK?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for ALK
Wild-type and mutant forms of ALK for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
ALK, Active
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-A19-11G
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~90 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: ALK Assays
What substrate does AssayQuant use for ALK kinase assays?
AssayQuant offers PhosphoSens peptide substrates optimized for ALK catalytic activity, derived from validated ALK phosphorylation sites. These Sox-based substrates produce a direct fluorescent signal upon phosphorylation, eliminating the need for antibodies or secondary detection reagents and enabling continuous real-time monitoring of kinase activity.
Can the PhosphoSens ALK assay measure inhibitor kinact/KI for covalent inhibitors?
Yes. Because PhosphoSens generates continuous fluorescent progress curves, time-dependent inhibition and covalent inactivation kinetics can be directly resolved from a single experiment. This allows straightforward determination of kinact and KI parameters that endpoint assays fundamentally cannot provide without laborious multi-timepoint designs.
How does physiological ATP concentration impact ALK inhibitor IC50 measurements?
Most ATP-competitive ALK inhibitors show strong ATP-competitive behavior, meaning IC50 values shift significantly with ATP concentration. By running assays at or near the cellular ATP Km, PhosphoSens provides IC50 values with greater predictive power for cellular and in vivo potency, avoiding the systematic underestimation of potency common with artificially low ATP conditions used in some endpoint formats.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ ALK Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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