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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About BMX
BMX (Bone Marrow X-linked kinase), also known as ETK, is a non-receptor tyrosine kinase belonging to the Tec family, which includes BTK, ITK, TEC, and TXK. Like other Tec kinases, BMX contains an N-terminal pleckstrin homology (PH) domain, a Tec homology (TH) domain, SH3 and SH2 domains, and a C-terminal catalytic kinase domain. BMX is activated downstream of PI3K-generated PIP3, which recruits BMX to the plasma membrane via its PH domain, facilitating transphosphorylation at Y566 in the activation loop. Key substrates include STAT3, AKT, and FAK. BMX participates in PI3K/AKT, JAK/STAT, and androgen receptor signaling pathways. It is highly expressed in hematopoietic progenitors, endothelial cells, and epithelial tissues. BMX has been implicated in prostate cancer progression, acute myeloid leukemia (AML), glioblastoma, and inflammatory signaling. Its role in promoting tumor cell survival, invasion, and resistance to apoptosis makes BMX a compelling oncology drug target, particularly in castration-resistant prostate cancer and hematologic malignancies.
Why PhosphoSens for BMX?
BMX presents several assay challenges in drug discovery: its requirement for physiological ATP concentrations (1-5 mM intracellularly) means that endpoint assays using low ATP can overestimate inhibitor potency, particularly for ATP-competitive compounds. ADP-Glo and HTRF detect single time-point signals, missing biphasic kinetics, substrate depletion artifacts, and covalent inhibitor mechanisms critical for BTK-class covalent probes targeting BMX C496. Radiometric assays add safety and throughput limitations. PhosphoSens continuous fluorescent assays use CSox-based peptide substrates to monitor BMX-catalyzed phosphorylation in real time at physiological ATP, generating full progress curves. This enables accurate Km(ATP) determination, detection of covalent kinact/KI parameters for irreversible inhibitors, and identification of time-dependent inhibition — all in a homogeneous, mix-and-read format compatible with high-throughput screening and mechanistic profiling.
Key Pathways:
PI3K/AKT/mTOR signaling
JAK/STAT3 signaling
Androgen receptor signaling
NF-kB inflammatory signaling
Talk to a Scientist
Have questions about BMX assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time BMX Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative BMX activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0794
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0794)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0794
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for BMX?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF BMX Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative BMX activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0515
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0515)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0515
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for BMX?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for BMX
Wild-type and mutant forms of BMX for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
BMX, Active Full Length
Rat • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-B07-10CG
- Mutation: Full Length
- Species: Rat
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~110 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: BMX Assays
How does PhosphoSens measure BMX kinase activity in real time?
PhosphoSens uses proprietary CSox-containing peptide substrates that undergo a fluorescence change upon phosphorylation by BMX, enabling continuous monitoring of kinase activity without stopping the reaction. This generates full progress curves at each inhibitor concentration, providing richer kinetic data than endpoint methods. The approach is antibody-free, homogeneous, and compatible with both purified BMX and cell lysate formats.
Can PhosphoSens assays detect covalent BMX inhibitors?
Yes u2014 because PhosphoSens monitors phosphorylation continuously over time, it directly captures the time-dependent loss of BMX activity characteristic of covalent inhibitors targeting C496, the equivalent of the BTK C481 covalent warhead site. Full progress curve analysis allows extraction of kinact and KI parameters that endpoint assays cannot resolve. This is particularly valuable for profiling irreversible Tec family inhibitors under development for BMX-driven cancers.
Why is physiological ATP concentration important for BMX inhibitor assays?
BMX inhibitor potency is strongly influenced by ATP concentration because most small-molecule inhibitors compete with ATP for the kinase active site. Assays run at artificially low ATP concentrations overestimate inhibitor affinity relative to cellular conditions where ATP is present at 1-5 mM. PhosphoSens assays are validated at physiological ATP concentrations, ensuring that Ki and IC50 values better predict cellular and in vivo inhibitor efficacy.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ BMX Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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