PhosphoSens EGFR Protein Kinase Assays, Substrates & Recombinant Enzymes

Continuous, real-time assays that directly quantify EGFR catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.

  • Real-time kinetic or TRF endpoint formats
  • Optimized substrates and assay-ready kits
  • Compatible with physiological ATP and cofactors

Designed for: drug discovery, lead optimization, and mechanistic kinase research teams

 

EGFR Kinase Activity Assays | Continuous, Direct Measurement | AssayQuant

How pricing works:
How pricing works: Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.

Real-Time EGFR Activity Assays

Continuous, real-time fluorescent assays optimized for quantitative EGFR activity measurements, IC50 determination, and mechanistic studies.

Assay Format: Continuous kinetic fluorescence (real-time)

PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.

Learn more about PhosphoSens-Kinetic →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Kinetic Kinase Activity Kit - AQT0734

Ready-to-use assay kits containing substrate and all essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0734)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $425

Substrate

PhosphoSens Kinase Substrate AQT0734

Bulk PhosphoSens® substrate for assay development and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for EGFR?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

Request a Quote

TRF EGFR Screening Assays

Robust endpoint TRF assays optimized for high-throughput screening and quantitative EGFR activity measurement in plate-based workflows.

Assay Format: Time-resolved fluorescence (TRF, endpoint)

PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.

Learn more about PhosphoSens-Red →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Red Kinase Activity Kit - AQT0653

Ready-to-use TRF assay kits containing substrate and essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0653)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
  • Europium Detection Solution

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $455

Substrate

PhosphoSens Kinase Substrate AQT0653

Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for EGFR?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts. Request a Quote

Active Recombinant Enzymes for EGFR

Wild-type and mutant forms of EGFR for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.

EGFR, Active

Human • Baculovirus-Insect Cells

Technical Specifications
  • Catalog: AQT-E10-112G
  • Species: Human
  • Expression System: Baculovirus-Insect Cells
  • Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
  • Molecular Weight: ~89-100kDa
  • Purity/Activity: For specific information on a given lot, see related technical data sheet.
  • Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:

Price: $ $348.00

Request a Quote

How many assays can I run per 1 mg of Sensor Peptide?

PhosphoSens Sensor Peptides range in molecular weight (MW) based on the size and sequence of the substrate. The average MW or a sensor peptide is 2,150 g/mol. This size Sensor Peptide will be re-suspended in approximately 450 µL of diluent. If running an assay in a 384-well plate, with a final well volume of 20 µL and Sensor Peptide concentration of 10 µM, you can run 2,250 assays!

How do I resuspend my lyophilized sensor peptide?

Reference the "Solubilization" section of the printed Certificate of Analysis (CofA) that is printed and shipped with every order. If you do not see this, please contact us at support@assayquant.com and we will share an electronic copy of your CofA. 

What is a reagent pack?

PhosphoSens Reagent Packs include reaction reagents to run PhosphoSens kinase and phosphatase assays under AssayQuant’s validated reaction conditions. Reagent packs are designed to pair seamlessly with PhosphoSens substrates, eliminating the need to source or optimize individual components.

Each reagent pack is formulated and volume-matched to support 2,000 assays in a 384-well plate format at a 25 µL reaction volume per well, ensuring consistent performance across screening, profiling, and mechanistic studies.

Components:
  • ATP Solution — 100 mM, 0.6 mL
  • DTT Solution — 1 M, 0.1 mL
  • Enzyme Reaction Buffer — 10× concentrate, 6 mL
  • Enzyme Dilution Buffer — 5× concentrate, 16 mL
  • EGTA Solution — 550 mM, 0.1 mL
  • Europium (III) Detection Reagent — 30 mM, 12 mL
    (PhosphoSens-Red format only)

All reagents are optimized for compatibility with PhosphoSens sensor peptide substrates and are quality-controlled to ensure reproducible signal, assay stability, and lot-to-lot consistency.

Applications:
  • Bulk substrate workflows
  • High-throughput screening (HTS)
  • Mechanistic and kinetic studies

When purchasing PhosphoSens assay kits, all required reagents are already included. Reagent packs are intended for use when substrates or enzymes are purchased separately or when scaling beyond kit formats.

Data & Resources

Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.

AQT0734 - EGFR Assay Validation Report

AQT0734 - EGFR Assay Validation Report

Each validation report provides experimental conditions and data showing:

  • Enzyme titration and linearity
  • Sensor peptide substrate titration and Km determination
  • ATP titration and Km determination
  • DMSO titration and assay tolerance
  • Inhibitor titration and IC50 determination
Download the Report

Protocol

Inhibitor IC50 Protocol

See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.

Download Protocol →

Webinar

Webinar: Continuous Assay Validation

Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.

Watch the Webinar →

Still have questions about our products?

Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.

Talk to a Scientist

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