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PhosphoSens CDK1/CycE1 Protein Kinase Assays, Substrates & Recombinant Enzymes
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About CDK1/CycE1
Cyclin-dependent kinase 1 (CDK1) is the founding and most essential member of the CDK family, functioning as the master regulator of mitotic entry and progression in eukaryotic cells. CDK1 is catalytically inactive as a monomer and requires association with cyclin partners — primarily Cyclin B1/B2 for M-phase entry and Cyclin E1 for S-phase regulation — as well as activating phosphorylation by CDK-activating kinase (CAK) at Thr161. CDK1/CyclinE1 complexes contribute to G1/S transition control, licensing origin firing and coordinating with CDK2 functions. Key substrates include Rb family members, Lamins, condensin, separase inhibitory proteins, and numerous centrosomal and spindle assembly factors. CDK1 sits at the convergence of the RB/E2F, DNA damage checkpoint, and Wnt signaling pathways. Dysregulation of CDK1 activity — through cyclin overexpression, loss of CIP/KIP inhibitors, or checkpoint abrogation — is pervasive across solid tumors and hematologic malignancies, making CDK1 a compelling oncology drug target whose inhibition forces mitotic catastrophe in cancer cells.
Why PhosphoSens for CDK1/CycE1?
CDK1/CyclinE1 drug discovery presents distinct challenges: the kinase shares high structural homology with CDK2 requiring exquisite selectivity profiling, and its activity is tightly regulated by phosphorylation state and cyclin stoichiometry, making endpoint assays prone to artifacts from incomplete reaction capture. ADP-Glo and HTRF endpoint formats miss burst-phase kinetics and covalent engagement, while radiometric methods lack throughput and real-time resolution. PhosphoSens continuous fluorescent kinase assays monitor CDK1/CyclinE1 substrate phosphorylation in real time at physiological ATP concentrations, generating full progress curves that enable accurate Km(ATP) determination, IC50 profiling without fixed-time bias, and direct measurement of covalent inhibitor kinact/KI parameters — critical for next-generation covalent CDK programs — all in a homogeneous, no-antibody, mix-and-read format compatible with HTS.
Key Pathways:
Cell cycle G1/S and G2/M checkpoint
RB/E2F transcriptional regulation
DNA damage response
Wnt/beta-catenin signaling
Talk to a Scientist
Have questions about CDK1/CycE1 assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time CDK1/CycE1 Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative CDK1/CycE1 activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0297
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0297)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0297
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CDK1/CycE1?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF CDK1/CycE1 Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative CDK1/CycE1 activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0296
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0296)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0296
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CDK1/CycE1?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteNo recombinant enzymes are currently available for CDK1/CycE1.
Frequently Asked Questions: CDK1/CycE1 Assays
What substrate does AssayQuant use for CDK1/CyclinE1 activity assays?
AssayQuant offers CSox-based peptide substrates engineered from validated CDK1 phosphorylation consensus sequences, incorporating the chelation-enhanced fluorophore that generates a real-time fluorescent signal upon phosphorylation. These substrates are optimized for CDK1/CyclinE1 to provide high signal-to-background ratios and Km values reflective of physiological substrate affinity. Contact our team for substrate and assay kit recommendations specific to your program.
Can the PhosphoSens assay measure CDK1/CyclinE1 inhibitor selectivity against CDK2?
Yes. Because PhosphoSens assays use the same continuous fluorescence readout platform across CDK family members, researchers can run parallel selectivity profiling of CDK1/CyclinE1 versus CDK2/CyclinE1 or CDK2/CyclinA2 under identical, physiologically relevant ATP conditions. This eliminates the inter-assay variability inherent in comparing endpoint formats, providing cleaner selectivity windows and more reliable SAR data.
How does the PhosphoSens platform handle covalent CDK1 inhibitor characterization?
PhosphoSens continuous progress curves capture the time-dependent inhibition signature characteristic of covalent inhibitors, allowing direct extraction of kinact and KI from a single experiment without specialized equipment or radioactivity. This is a significant advantage over ADP-Glo or HTRF endpoint assays, which systematically underestimate or miss covalent engagement depending on incubation time chosen, and is critical for advancing irreversible or pseudo-irreversible CDK1 inhibitor scaffolds.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ CDK1/CycE1 Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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