AQT0515 PhosphoSens® Tyrosine Kinase Substrate
A PhosphoSens® tyrosine kinase substrate validated for 38 kinases (37 wild-type, 1 mutant), including FGFR1, FLT3, LCK. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0515
- Enzyme class
- Kinase
- Residue specificity
- Tyrosine
- Validated kinases
- 38
- Assay formats
- Kinetic · 1 Red · 38
- Kinase families
- 14
Kinases validated with AQT0515
Every kinase below has a validated PhosphoSens® assay using AQT0515. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0515 | Link |
|---|---|---|---|---|
| ALK [L1196M/G1202R] | Mutant | ALK | Red | View assay → |
| BLK | Wild-type | Src | Red | View assay → |
| BMX | Wild-type | Tec | Red | View assay → |
| EPHA1 | Wild-type | Eph | Red | View assay → |
| EPHA3 | Wild-type | Eph | Red | View assay → |
| EPHA4 | Wild-type | Eph | Red | View assay → |
| EPHA5 | Wild-type | Eph | Red | View assay → |
| EPHB1 | Wild-type | Eph | Red | View assay → |
| EPHB2 | Wild-type | Eph | Red | View assay → |
| EPHB3 | Wild-type | Eph | Red | View assay → |
| EPHB4 | Wild-type | Eph | Red | View assay → |
| FER | Wild-type | Fer | Red | View assay → |
| FES | Wild-type | Fer | Red | View assay → |
| FGFR1 | Wild-type | FGFR | Red | View assay → |
| FGFR2 | Wild-type | FGFR | Red | View assay → |
| FGFR3 | Wild-type | FGFR | Red | View assay → |
| FGR | Wild-type | Src | Red | View assay → |
| FLT3 | Wild-type | PDGFR | Red | View assay → |
| FYN (FYNa) | Wild-type | Src | Red | View assay → |
| FYN (FYNb) | Wild-type | Src | Red | View assay → |
| HCK | Wild-type | Src | Red | View assay → |
| IGF1R | Wild-type | InsR | Red | View assay → |
| INSR | Wild-type | InsR | Red | View assay → |
| ITK | Wild-type | Tec | Red | View assay → |
| LCK | Wild-type | Src | Red | View assay → |
| LYN (LYNa) | Wild-type | Src | Red | View assay → |
| MERTK | Wild-type | Axl | Red | View assay → |
| NTRK1 | Wild-type | Trk | Red | View assay → |
| NTRK2 | Wild-type | Trk | Red | View assay → |
| NTRK3 | Wild-type | Trk | Red | View assay → |
| PDGFRB | Wild-type | PDGFR | Red | View assay → |
| PTK2B | Wild-type | FAK | Red | View assay → |
| RET | Wild-type | Ret | Red | View assay → |
| ROS1 | Wild-type | Sev | Red | View assay → |
| TNK1 | Wild-type | Ack | Red | View assay → |
| TNK2 | Wild-type | Ack | KineticRed | View assay → |
| TYRO3 | Wild-type | Axl | Red | View assay → |
| YES1 | Wild-type | Src | Red | View assay → |
No kinases match that search.
Bulk substrate
AQT0515 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0515 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 38 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0515 validated for?
AQT0515 is validated for 38 kinases (37 wild-type and 1 mutant), including FGFR1, FLT3, LCK. See the full list above.
Which PhosphoSens assay formats use AQT0515?
AQT0515 is the PhosphoSens-Kinetic substrate for 1 kinase. It is the PhosphoSens-Red substrate for 38 kinases.
What sizes is AQT0515 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.