PhosphoSens FGFR1 Protein Kinase Assays, Substrates & Recombinant Enzymes
Continuous, real-time assays that directly quantify FGFR1 catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.
Real-time kinetic or TRF endpoint formats
Optimized substrates and assay-ready kits
Compatible with physiological ATP and cofactors
Designed for: drug discovery, lead optimization, and mechanistic kinase research teams
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously
monitoring substrate phosphorylation or dephosphorylation in real time,
generating a full progress curve in every well.
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection
to enable robust, plate-based quantification of enzyme activity, making them
well suited for high-throughput screening workflows.
Wild-type and mutant forms of FGFR1 for assay development, kinase profiling, and mechanistic studies.
Enzymes are supplied active and optimized for PhosphoSens® substrates.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Storage Buffer: Supplied as sterile recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Molecular Weight: 74 kDa
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Storage Buffer: Supplied as sterile recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Molecular Weight: 74 kDa
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
FGFR1 (C488A C584A), Active
N-term, C488A and C584A
Human
• Baculovirus-Insect Cells
Technical Specifications
Catalog: AQT-F04-12CG
Mutation: N-term, C488A and C584A
Species: Human
Expression System: Baculovirus-Insect Cells
Storage Buffer: Supplied as sterile recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Molecular Weight: 74 kDa
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample
data, protocols and other resources to see how our assays perform and to help you get started in your own lab.
All validation data generated using PhosphoSens® assays under recommended conditions.
Discover how continuous assay formats power deep understanding of kinase function. See how
PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and
mechanistic characterization.