PhosphoSens is a continuous, real-time fluorescence assay that tracks phosphatase-catalyzed dephosphorylation as it happens, well by well, rather than reading a single timepoint. It uses substrates built around native phosphosite sequences instead of a generic fluorogenic reporter like DiFMUP, so the signal reflects true catalytic turnover on a physiologically relevant substrate.

TDI compounds produce a characteristic change in the progress curve shape — the inhibition deepens over time as the compound slowly occupies or covalently modifies the enzyme. Because PhosphoSens monitors activity continuously, this curve deviation is directly visible. Endpoint assays that measure at a single time point will either miss TDI entirely or mischaracterize its potency, depending on when they sample the reaction.

We offer custom assay development for kinase targets not currently in our catalog. Our team can design and validate a PhosphoSens substrate for your target, typically within 8–12 weeks. Contact us to discuss your target, timeline, and project requirements.

 Yes — KinSight Profiling now includes a phosphatase panel as a full CRO service. Send in your compounds and receive an annotated kinetic report. 

Phosphatase selectivity profiling measures how a compound affects the catalytic activity of protein phosphatases across a panel of related enzymes — not just one target in isolation. Because phosphatase active sites can share structural features across families, a compound optimized against one target can retain unwanted activity against others. Profiling maps that activity directly, rather than inferring it from binding alone.

 The catalog includes targets across the PPP (Ser/Thr), PPM (Mg²⁺/Mn²⁺-dependent), PTPN (non-receptor tyrosine), PTPR (receptor-type tyrosine), and DUSP (dual-specificity) phosphatase families, among others, covering 50 validated targets in total.