AQT0523 PhosphoSens® Phosphatase Substrate

A PhosphoSens® phosphatase substrate validated for 10 phosphatases, including CDC25A, PPP2CA, CDC25B. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.

Catalog number
AQT0523
Enzyme class
Phosphatase
Validated phosphatases
10
Assay formats
Kinetic · 10
Phosphatase families
4

Phosphatases validated with AQT0523

Every phosphatase below has a validated PhosphoSens® assay using AQT0523. Select a target to see its kits, recombinant enzyme, pricing and protocols.

PhosphataseTypeFamilyFormat with AQT0523Link
CDC25A Wild-type CDC25 Kinetic View assay →
CDC25B Wild-type CDC25 Kinetic View assay →
CDC25C Wild-type CDC25 Kinetic View assay →
DUSP1 Wild-type DUSP (dual-specificity phosphatase) Kinetic View assay →
DUSP6 Wild-type DUSP (dual-specificity phosphatase) Kinetic View assay →
PPM1F Wild-type PPM (Mg2+/Mn2+-dependent Ser/Thr phosphatase) Kinetic View assay →
PPM1H Wild-type PPM (Mg2+/Mn2+-dependent Ser/Thr phosphatase) Kinetic View assay →
PPP1CB Wild-type PPP (Ser/Thr phosphatase) Kinetic View assay →
PPP2CA Wild-type PPP (Ser/Thr phosphatase) Kinetic View assay →
PPP2CB Wild-type PPP (Ser/Thr phosphatase) Kinetic View assay →

Bulk substrate

AQT0523 PhosphoSens® Phosphatase Substrate

Bulk substrate for assay development, screening and high-throughput workflows. Phosphatase assay kits that include AQT0523 are on each target's assay page.

Price: $2895 USD

How PhosphoSens® substrates work

PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the phosphatase removes the phosphate, fluorescence decreases, so activity is read continuously and directly in a plate reader.

  • Real-time progress curves, not single end-points
  • No antibodies, coupling enzymes, wash steps or radioactivity
  • One substrate across 10 phosphatases simplifies selectivity and panel work
Learn about PhosphoSens® technology →

Frequently asked questions

Which phosphatases is AQT0523 validated for?

AQT0523 is validated for 10 phosphatases, including CDC25A, PPP2CA, CDC25B. See the full list above.

Which PhosphoSens assay formats use AQT0523?

AQT0523 is the PhosphoSens-Kinetic substrate for 10 phosphatases.

What sizes is AQT0523 available in?

1 mg, 5 mg, 10 mg and 20 mg, priced from $2895 (1 mg) to $38625 (20 mg) USD.

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