PhosphoSens PTPN2 Protein Phosphatase Assays, Substrates & Recombinant Enzymes

Continuous, real-time assays that directly quantify PTPN2 catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.

  • Real-time kinetic or TRF endpoint formats
  • Optimized substrates and assay-ready kits
  • Compatible with physiological ATP and cofactors

Designed for: drug discovery, lead optimization, and mechanistic kinase research teams

 

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PhosphoSens PTPN2 kinase assay kit — continuous real-time fluorescence

How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.

Real-Time PTPN2 Activity Assays

Continuous, real-time fluorescent assays optimized for quantitative PTPN2 phosphatase activity measurements, IC50 determination, and mechanistic studies.

Assay Format: Continuous phosphatase fluorescence (real-time)

PhosphoSens-Kinetic assays directly quantify phosphatase activity by continuously monitoring substrate dephosphorylation in real time, generating a full progress curve in every well.

Learn more about PhosphoSens-Kinetic →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Kinetic Phosphatase Activity Kit - AQT0264

Ready-to-use assay kits containing substrate and all essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0266)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $450

Substrate

PhosphoSens Phosphatase Substrate AQT0264

Bulk PhosphoSens® substrate for assay development and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2895

Ready to request pricing for PTPN2?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

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No PhosphoSens-Red format is currently available for PTPN2.

Ready to request pricing for PTPN2?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

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Active Recombinant Enzymes for PTPN2

Wild-type and mutant forms of PTPN2 for assay development, phosphatase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.

PTPN2 (TC-PTP), Active Full Length

Human • E. coli

Technical Specifications
  • Catalog: AQT-P29-20G
  • Mutation: Full Length
  • Species: Human
  • Expression System: E. coli
  • Storage Buffer: Supplied as sterile 20 mM MOPS, pH 7.5, 300mM NaCl, 10mM glutathione, 0.25-1mM DTT, 0-0.1mM PMSF, 10-25% glycerol
  • Molecular Weight: ~69 kDa
  • Purity/Activity: For specific information on a given lot, see related technical data sheet.
  • Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:

Price: $$248.00

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🔬 150+ peer-reviewed citations · 🏛️ MIT spinout technology · ⚗️ PhosphoSens® phosphatase assays validated by independent labs · Browse technical articles →

Data & Resources

Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.

Sample Report: AQT0734 - EGFR Assay Validation Report

Sample Report: AQT0734 - EGFR Assay Validation Report

Each validation report provides experimental conditions and data showing:

  • Enzyme titration and linearity
  • Sensor peptide substrate titration and Km determination
  • ATP titration and Km determination
  • DMSO titration and assay tolerance
  • Inhibitor titration and IC50 determination
Download the Report

Protocol

Inhibitor IC50 Protocol

See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.

Download Protocol →

Webinar

Webinar: Continuous Assay Validation

Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.

Watch the Webinar →

Service

PTPN2 Phosphatase Profiling

Need custom profiling data? Our team can run your compounds against PTPN2 and related phosphatases under identical PhosphoSens conditions.

Learn about Phosphatase Profiling →

Still have questions about our products?

Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.

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