.jpg?width=450&name=PhosphoSens%20Packaging%20C%20(3).jpg)
Still have questions about our products?
Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About CAMK2D
CAMK2D (CaMKII delta) is a member of the calcium/calmodulin-dependent protein kinase II family, a multifunctional serine/threonine kinase family that forms dodecameric holoenzymes via their C-terminal association domains. Activation is triggered by Ca2+/calmodulin binding, which relieves autoinhibition and enables trans-autophosphorylation at Thr287, locking the kinase in a calcium-independent, constitutively active state. CAMK2D is the predominant CaMKII isoform in the heart and is a central regulator of excitation-contraction coupling, phosphorylating key substrates including phospholamban (PLN), ryanodine receptor 2 (RYR2), and histone deacetylase 4 (HDAC4). These phosphorylation events modulate SR calcium handling, gene transcription, and cardiomyocyte hypertrophy. Dysregulated CAMK2D activity is strongly implicated in heart failure, atrial fibrillation, and cardiac arrhythmias. Its role in pathological cardiac remodeling and pro-arrhythmic calcium leak makes CAMK2D an attractive drug target for cardiovascular disease, with emerging interest in its roles in neurological and oncological contexts.
Why PhosphoSens for CAMK2D?
CAMK2D presents significant assay challenges due to its requirement for Ca2+/calmodulin co-activation, its oligomeric holoenzyme architecture, and its propensity for autophosphorylation that can confound endpoint readouts. Traditional ADP-Glo and HTRF assays capture only a single time point, masking autophosphorylation artifacts and failing to distinguish covalent or slow-binding inhibitors from competitive ones. Radiometric assays add radioactive waste concerns and low throughput. PhosphoSens continuous kinetic assays resolve these issues by generating full progress curves in real time under physiological ATP concentrations, enabling accurate Km(ATP) determination and rigorous kinact/KI characterization for irreversible inhibitors. Real-time monitoring detects autophosphorylation-driven baseline drift and allows researchers to define the true linear phase, dramatically improving data quality for CAMK2D drug discovery campaigns.
Key Pathways:
Ca2+/CaM Signaling
Cardiac Excitation-Contraction Coupling
HDAC-MEF2 Transcriptional Regulation
CaMKII Autophosphorylation Cascade
Talk to a Scientist
Have questions about CAMK2D assay design, selectivity panels, or covalent inhibitor characterization?
Ask Our Scientists →
How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time CAMK2D Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative CAMK2D activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0425
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0425)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0425
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CAMK2D?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF CAMK2D Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative CAMK2D activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0425
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0425)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0425
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CAMK2D?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteNo recombinant enzymes are currently available for CAMK2D.
Frequently Asked Questions: CAMK2D Assays
How does PhosphoSens handle the Ca2+/calmodulin activation requirement for CAMK2D assays?
PhosphoSens peptide substrates are compatible with assay buffers containing defined Ca2+/calmodulin concentrations, allowing CAMK2D to be assayed in its activated state. Real-time fluorescence monitoring immediately detects activity upon Ca2+/CaM addition, enabling dose-response characterization of activator dependence without endpoint sampling artifacts.
Can PhosphoSens assays distinguish CAMK2D autophosphorylation from substrate phosphorylation?
Yes. Because PhosphoSens monitors phosphorylation of a specific exogenous peptide substrate in real time, autophosphorylation of the enzyme itself does not generate a fluorescent signal from the substrate sensor. This allows researchers to isolate substrate phosphorylation kinetics and clearly identify when autophosphorylation-driven enzyme activation influences the progress curve shape.
Is the PhosphoSens assay suitable for characterizing slow-binding or covalent CAMK2D inhibitors?
PhosphoSens continuous kinetics are ideally suited for this purpose, as full progress curves reveal the characteristic time-dependent inhibition signatures of slow-on/slow-off and covalent inhibitors that are invisible to single-endpoint assays like ADP-Glo or HTRF. The platform enables direct determination of kinact and KI values without additional secondary assay formats.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ CAMK2D Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
Want to hear the latest about our technology? Be among the first to learn about our latest products and services.