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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About AURKA
Aurora Kinase A (AURKA) is a serine/threonine kinase belonging to the Aurora family, which also includes AURKB and AURKC. AURKA localizes to centrosomes and spindle poles, where it orchestrates mitotic entry, centrosome maturation, bipolar spindle assembly, and cytokinesis. Activation requires autophosphorylation at Thr288 within the activation loop, potentiated by binding partners including TPX2, which shields Thr288 from PP1-mediated dephosphorylation. Key substrates include LATS2, PLK1, BRCA1, CDC25B, and Eg5, linking AURKA to G2/M checkpoint regulation and DNA damage response. AURKA is amplified or overexpressed in breast, colorectal, ovarian, pancreatic, and hematologic malignancies, driving chromosomal instability and resistance to taxane-based therapy. Its role in MYC stabilization via phosphorylation of MYCN at Ser62 has expanded interest to neuroblastoma and other MYC-driven cancers. These oncogenic properties, combined with a well-defined ATP-binding pocket, make AURKA a compelling kinase drug target with multiple clinical-stage inhibitors under evaluation.
Why PhosphoSens for AURKA?
Profiling AURKA presents several assay challenges: low intrinsic activity without activator peptides (e.g., TPX2-derived), autophosphorylation artifacts at Thr288 that confound endpoint readouts, and the need to distinguish substrate phosphorylation from background kinase autophosphorylation. ADP-Glo and HTRF endpoint assays capture only a single timepoint, masking burst kinetics and autophosphorylation contributions that inflate apparent activity. Radiometric assays lack the throughput needed for modern inhibitor profiling. AssayQuant's PhosphoSens CSox-based peptide substrates enable continuous, real-time fluorescence monitoring of AURKA activity at physiological ATP concentrations, generating full progress curves that resolve autophosphorylation from substrate turnover. This approach enables accurate Ki and kinact/KI determination for covalent inhibitors, allows TPX2 activation studies under steady-state conditions, and eliminates endpoint artifacts — dramatically improving data quality for SAR campaigns and selectivity profiling.
Key Pathways:
Mitotic spindle assembly checkpoint
G2/M cell cycle transition
DNA damage response
MYC oncogenic signaling
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Have questions about AURKA assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time AURKA Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative AURKA activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0186
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0186)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0186
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for AURKA?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF AURKA Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative AURKA activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0186
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0186)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0186
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for AURKA?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for AURKA
Wild-type and mutant forms of AURKA for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
AURORA A, Active Full Length
Mouse • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-A28-10G
- Mutation: Full Length
- Species: Mouse
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~71 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: AURKA Assays
How does PhosphoSens measure AURKA activity in real time?
PhosphoSens uses proprietary CSox-based peptide substrates that incorporate a chelation-enhanced fluorophore adjacent to the phosphorylation site; upon phosphorylation by AURKA, the fluorescence signal changes continuously and proportionally to product formation. This generates a complete progress curve from a single reaction well, enabling calculation of initial velocities, IC50 values, and kinetic constants without stopping the reaction or adding detection reagents.
Can the AURKA assay be run with TPX2 activator peptide to reflect physiological activation?
Yes, AssayQuant's AURKA assay format is compatible with addition of TPX2-derived activator peptides, allowing researchers to study inhibitor potency against the fully activated, physiologically relevant form of the kinase. Running real-time progress curves with and without TPX2 also enables direct quantification of the fold-activation and helps distinguish compounds that disrupt the AURKA-TPX2 interaction from those targeting the ATP-binding site.
Why is a continuous kinase assay preferred over ADP-Glo for AURKA inhibitor profiling?
AURKA undergoes rapid autophosphorylation at Thr288 that can consume ATP and confound single-endpoint ADP-Glo measurements, leading to overestimation of substrate phosphorylation or underestimation of inhibitor potency. PhosphoSens continuous assays capture the full kinetic trajectory, allowing researchers to distinguish autophosphorylation from substrate turnover and to identify non-linear kinetics u2014 artifacts that endpoint assays miss entirely.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ AURKA Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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