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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About AURKB
Aurora Kinase B (AURKB) is a serine/threonine kinase and founding member of the chromosomal passenger complex (CPC), which also includes INCENP, Survivin, and Borealin. AURKB is activated through autophosphorylation at Thr232 and allosteric stimulation by INCENP, reaching peak activity during mitosis. Key substrates include histone H3 (Ser10 and Ser28), MCAK, MIS12, and KIF2C, coordinating chromosome condensation, kinetochore-microtubule attachments, and cytokinesis. AURKB governs the spindle assembly checkpoint by correcting erroneous kinetochore attachments, ensuring faithful chromosome segregation. Dysregulation of AURKB drives chromosomal instability (CIN) and aneuploidy, hallmarks of cancer. AURKB is overexpressed in colorectal, breast, lung, hematologic, and gastric malignancies and correlates with poor prognosis. Its essential mitotic role, combined with frequent overexpression in tumors, makes AURKB an attractive oncology drug target, with selective inhibition inducing mitotic catastrophe and tumor cell death while offering a therapeutic window over normal tissues.
Why PhosphoSens for AURKB?
AURKB assay development is complicated by its obligate dependence on the INCENP activator fragment for full catalytic activity, requiring careful co-reconstitution to avoid underestimating inhibitor potency. Endpoint methods such as ADP-Glo and HTRF require fixed ATP concentrations that deviate from physiological levels, distorting Ki measurements for ATP-competitive inhibitors and missing covalent or slow-binding mechanisms. Radiometric assays provide sensitivity but create throughput and safety bottlenecks. AssayQuant's PhosphoSens CSox-based peptide substrates enable continuous, real-time fluorescent monitoring of AURKB phosphorylation at physiological ATP concentrations, generating full progress curves that faithfully capture kinact/KI for covalent inhibitors, residence time effects, and authentic Km/Vmax parameters. This eliminates endpoint artifacts, reduces assay variability, and accelerates SAR campaigns against this critical mitotic kinase.
Key Pathways:
Spindle Assembly Checkpoint
Chromosomal Passenger Complex Signaling
Cell Cycle Regulation (M-phase)
Histone H3 Phosphorylation Pathway
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time AURKB Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative AURKB activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0186
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0186)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0186
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
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Request a QuoteTRF AURKB Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative AURKB activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0186
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0186)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0186
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for AURKB?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for AURKB
Wild-type and mutant forms of AURKB for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
AURORA B (G160L), Active Full Length, G160L
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-A31-10BG
- Mutation: Full Length, G160L
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~68 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$338.00
Request a QuoteFrequently Asked Questions: AURKB Assays
Why does AURKB require INCENP for robust kinase assay activity?
AURKB autophosphorylation alone yields submaximal activity; the IN-box domain of INCENP binds the AURKB activation loop and dramatically stimulates catalytic turnover by stabilizing the active conformation. Omitting INCENP from assay reconstitution results in poor signal, underestimation of Vmax, and artifactually weak potency readouts for ATP-competitive inhibitors. AssayQuant's AURKB reagents are optimized with the appropriate INCENP co-activator fragment to ensure physiologically relevant activity.
How does PhosphoSens technology improve AURKB inhibitor potency measurements compared to ADP-Glo?
ADP-Glo requires non-physiological ATP concentrations and fixed reaction endpoints, which distort IC50 values for ATP-competitive AURKB inhibitors according to the Cheng-Prusoff relationship and mask time-dependent inhibition. PhosphoSens continuous assays monitor substrate phosphorylation in real time using a fluorescent CSox sensor, enabling IC50 and Ki determinations at physiological ATP levels and revealing slow-on/slow-off kinetics or covalent mechanisms that endpoint formats cannot detect.
Can the PhosphoSens AURKB assay be used to profile selectivity against Aurora Kinase A?
Yes. AssayQuant offers parallel PhosphoSens assay kits for both AURKA and AURKB using matched CSox-peptide substrates and standardized assay conditions, enabling direct head-to-head selectivity profiling under identical ATP concentrations and buffer conditions. This side-by-side format is critical for advancing selective AURKB inhibitors that avoid AURKA-mediated toxicity, and the continuous readout captures any differential binding kinetics between the two isoforms.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ AURKB Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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