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About AURKC
Aurora kinase C (AURKC) belongs to the Aurora serine/threonine kinase family, which includes AURKA and AURKB. AURKC is predominantly expressed in the testes and is essential for male meiosis, regulating chromosome segregation and spindle assembly during spermatogenesis. It shares structural and functional homology with AURKB and can substitute for AURKB in the chromosomal passenger complex (CPC), interacting with INCENP, Survivin, and Borealin. Key substrates include histone H3 (Ser10/Ser28), INCENP, and MKlp2. AURKC participates in pathways governing mitotic fidelity, cytokinesis, and spindle checkpoint activation. While less studied than AURKA or AURKB, AURKC overexpression has been detected in multiple human cancers including breast, liver, and colorectal tumors, where it may compensate for AURKB and drive chromosomal instability. Loss-of-function mutations in AURKC cause male infertility due to macrozoospermia. Its tissue-restricted normal expression and oncogenic overexpression profile make it an attractive cancer drug target with a potentially favorable therapeutic window.
Why PhosphoSens for AURKC?
AURKC drug discovery presents unique assay challenges due to its low endogenous expression outside testes, difficulty obtaining recombinant active enzyme, and the need to distinguish AURKC activity from the closely related AURKB. Traditional endpoint assays such as ADP-Glo and HTRF require quenched timepoints that miss early kinetic events, obscure compound interference with the substrate-enzyme interaction, and cannot capture covalent or slow-binding inhibitor mechanisms. Radiometric assays provide sensitivity but are low-throughput and hazardous. PhosphoSens continuous fluorescent kinase assays monitor substrate phosphorylation in real time using physiological ATP concentrations, generating full progress curves that reveal mechanistic nuance, accurately measure kinact/KI for covalent inhibitors, and eliminate endpoint artifacts. This enables robust IC50, Km, and selectivity profiling against AURKB under identical conditions, accelerating identification of AURKC-selective chemical matter relevant to cancer and male fertility research.
Key Pathways:
Chromosomal Passenger Complex signaling
Spindle Assembly Checkpoint
Meiotic chromosome segregation
Histone H3 phosphorylation
Talk to a Scientist
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time AURKC Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative AURKC activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0186
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0186)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0186
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
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Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF AURKC Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative AURKC activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0186
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0186)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0186
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for AURKC?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for AURKC
Wild-type and mutant forms of AURKC for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
AURORA C, Active Full Length
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-A33-10G
- Mutation: Full Length
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~61 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: AURKC Assays
How does PhosphoSens enable AURKC selectivity profiling against AURKB?
PhosphoSens continuous assays use the same peptide substrate under identical physiological ATP conditions for both AURKB and AURKC, allowing direct kinetic comparison in parallel reactions. Real-time progress curves reveal differences in compound potency and binding kinetics without endpoint-to-endpoint variability. This is critical for identifying inhibitors that discriminate between these highly homologous kinases.
Can PhosphoSens assays detect covalent or slow-binding AURKC inhibitors?
Yes. Because PhosphoSens monitors phosphorylation continuously, deviations from linear kinetics caused by covalent or slow-binding inhibitors are immediately visible in the progress curves. Full kinact/KI parameters for covalent inhibitors and kon/koff for slow binders can be extracted from a single experiment, capabilities not accessible with endpoint ADP-Glo or HTRF formats.
What ATP concentration is used in AssayQuant AURKC assays and why does it matter?
AssayQuant PhosphoSens assays are run at physiological ATP concentrations (approximating cellular ATP levels near 1 mM), which directly affects IC50 values for ATP-competitive inhibitors. Using physiological ATP yields IC50 data that more accurately predicts cellular potency compared to assays run at low ATP concentrations that artificially inflate apparent compound potency.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ AURKC Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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