AQT0186 PhosphoSens® Serine/threonine Kinase Substrate
A PhosphoSens® serine/threonine kinase substrate validated for 5 kinases (4 wild-type, 1 mutant), including AURKA, AURKB, AURKC. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0186
- Enzyme class
- Kinase
- Residue specificity
- Serine/threonine
- Validated kinases
- 5
- Assay formats
- Kinetic · 5 Red · 4
- Kinase families
- 2
Kinases validated with AQT0186
Every kinase below has a validated PhosphoSens® assay using AQT0186. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0186 | Link |
|---|---|---|---|---|
| AURKA | Wild-type | Aur | KineticRed | View assay → |
| AURKB | Wild-type | Aur | KineticRed | View assay → |
| AURKB [G160L] | Mutant | Aur | Kinetic | View assay → |
| AURKC | Wild-type | Aur | KineticRed | View assay → |
| PRKX | Wild-type | PKA | KineticRed | View assay → |
No kinases match that search.
Bulk substrate
AQT0186 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0186 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 5 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0186 validated for?
AQT0186 is validated for 5 kinases (4 wild-type and 1 mutant), including AURKA, AURKB, AURKC. See the full list above.
Which PhosphoSens assay formats use AQT0186?
AQT0186 is the PhosphoSens-Kinetic substrate for 5 kinases. It is the PhosphoSens-Red substrate for 4 kinases.
What sizes is AQT0186 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.