AQT0661 PhosphoSens® Tyrosine Kinase Substrate
A PhosphoSens® tyrosine kinase substrate validated for 22 kinases (18 wild-type, 4 mutant), including KIT, EPHA2, EPHA3. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0661
- Enzyme class
- Kinase
- Residue specificity
- Tyrosine
- Validated kinases
- 22
- Assay formats
- Kinetic · 22
- Kinase families
- 8
Kinases validated with AQT0661
Every kinase below has a validated PhosphoSens® assay using AQT0661. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0661 | Link |
|---|---|---|---|---|
| BCR-RET | Mutant | Ret | Kinetic | View assay → |
| EPHA2 | Wild-type | Eph | Kinetic | View assay → |
| EPHA3 | Wild-type | Eph | Kinetic | View assay → |
| EPHA4 | Wild-type | Eph | Kinetic | View assay → |
| EPHB1 | Wild-type | Eph | Kinetic | View assay → |
| EPHB2 | Wild-type | Eph | Kinetic | View assay → |
| EPHB3 | Wild-type | Eph | Kinetic | View assay → |
| EPHB4 | Wild-type | Eph | Kinetic | View assay → |
| FGR | Wild-type | Src | Kinetic | View assay → |
| INSRR | Wild-type | InsR | Kinetic | View assay → |
| JAK2 (JH1) | Wild-type | Jak | Kinetic | View assay → |
| JAK2 (JH1JH2) | Wild-type | Jak | Kinetic | View assay → |
| JAK3 (JH1) | Wild-type | Jak | Kinetic | View assay → |
| KIT | Wild-type | PDGFR | Kinetic | View assay → |
| PDGFRA | Wild-type | PDGFR | Kinetic | View assay → |
| PDGFRB | Wild-type | PDGFR | Kinetic | View assay → |
| PTK2B | Wild-type | FAK | Kinetic | View assay → |
| RET | Wild-type | Ret | Kinetic | View assay → |
| RET [G810R] | Mutant | Ret | Kinetic | View assay → |
| RET [G810S] | Mutant | Ret | Kinetic | View assay → |
| RET [V840M/G810S] | Mutant | Ret | Kinetic | View assay → |
| ROS1 | Wild-type | Sev | Kinetic | View assay → |
No kinases match that search.
Bulk substrate
AQT0661 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0661 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 22 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0661 validated for?
AQT0661 is validated for 22 kinases (18 wild-type and 4 mutant), including KIT, EPHA2, EPHA3. See the full list above.
Which PhosphoSens assay formats use AQT0661?
AQT0661 is the PhosphoSens-Kinetic substrate for 22 kinases.
What sizes is AQT0661 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.