Continuous, real-time assays that directly quantify KIT catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.
Real-time kinetic or TRF endpoint formats
Optimized substrates and assay-ready kits
Compatible with physiological ATP and cofactors
Designed for: drug discovery, lead optimization, and mechanistic kinase research teams
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously
monitoring substrate phosphorylation or dephosphorylation in real time,
generating a full progress curve in every well.
Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0661
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
Optimized PhosphoSens® substrate (AQT0661)
ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit.
View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0661
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate.
View Reagent Packs
Price: $2795
Ready to request pricing for KIT?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
No PhosphoSens-Red format is currently available for KIT.
Ready to request pricing for KIT?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Active Recombinant Enzymes for KIT
Wild-type and mutant forms of KIT for assay development, kinase profiling, and mechanistic studies.
Enzymes are supplied active and optimized for PhosphoSens® substrates.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$338.00
c-KIT (d557-558 V654A Y823D), Active
d557-558, V654A, Y823D
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$338.00
c-KIT (V560G V654A D820Y), Active
V560G, V654A, D820Y
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$338.00
🔬 150+ peer-reviewed citations·🏛️ MIT spinout technology·⚗️ PhosphoSens® validated by independent labs worldwide·Browse technical articles →
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample
data, protocols and other resources to see how our assays perform and to help you get started in your own lab.
All validation data generated using PhosphoSens® assays under recommended conditions.
Discover how continuous assay formats power deep understanding of kinase function. See how
PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and
mechanistic characterization.
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