AQT0734 PhosphoSens® Tyrosine Kinase Substrate

A PhosphoSens® tyrosine kinase substrate validated for 30 kinases (1 wild-type, 29 mutant), including EGFR. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.

Catalog number
AQT0734
Enzyme class
Kinase
Residue specificity
Tyrosine
Validated kinases
30
Assay formats
Kinetic · 30
Kinase families
2

Kinases validated with AQT0734

Every kinase below has a validated PhosphoSens® assay using AQT0734. Select a target to see its kits, recombinant enzyme, pricing and protocols.

KinaseTypeFamilyFormat with AQT0734Link
EGFR Wild-type EGFR Kinetic View assay →
EGFR (D770_N771insNPG) Mutant EGFR Kinetic View assay →
EGFR [A763_Y764insFHEA] Mutant EGFR Kinetic View assay →
EGFR [A763_Y764insFQEA] Mutant EGFR Kinetic View assay →
EGFR [A767-S768insTLA] Mutant EGFR Kinetic View assay →
EGFR [C775S/T790M/L858R] Mutant EGFR Kinetic View assay →
EGFR [C797S/L858R] Mutant EGFR Kinetic View assay →
EGFR [d746-750/T790M/C797S] Mutant EGFR Kinetic View assay →
EGFR [del746-750/C797S/T790M] Mutant EGFR Kinetic View assay →
EGFR [del746-750/C797S] Mutant EGFR Kinetic View assay →
EGFR [del746-750/T790M] Mutant EGFR Kinetic View assay →
EGFR [del746-750] Mutant EGFR Kinetic View assay →
EGFR [L718Q/L858R] Mutant EGFR Kinetic View assay →
EGFR [L718Q] Mutant EGFR Kinetic View assay →
EGFR [L858R] Mutant EGFR Kinetic View assay →
EGFR [L861Q] Mutant EGFR Kinetic View assay →
EGFR [N771-P772insH] Mutant EGFR Kinetic View assay →
EGFR [N771-P772insN] Mutant EGFR Kinetic View assay →
EGFR [S768I] Mutant EGFR Kinetic View assay →
EGFR [T790M/C797S/L858R/R999A] Mutant EGFR Kinetic View assay →
EGFR [T790M/C797S/L858R] Mutant EGFR Kinetic View assay →
EGFR [T790M/C797S] Mutant EGFR Kinetic View assay →
EGFR [T790M/L792F/C797S/L858R] Mutant EGFR Kinetic View assay →
EGFR [T790M/L792H/C797S/L858R] Mutant EGFR Kinetic View assay →
EGFR [T790M/L858R] Mutant EGFR Kinetic View assay →
EGFR [T790M] Mutant EGFR Kinetic View assay →
EGFR [V769-D770insGE] Mutant EGFR Kinetic View assay →
PDGFRA [D842V] Mutant PDGFR Kinetic View assay →
PDGFRA [T674I] Mutant PDGFR Kinetic View assay →
PDGFRA [V561D] Mutant PDGFR Kinetic View assay →

Bulk substrate

AQT0734 PhosphoSens® Kinase Substrate

Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0734 are on each target's assay page.

Price: $2795 USD

How PhosphoSens® substrates work

PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.

  • Real-time progress curves, not single end-points
  • Run at physiologically relevant ATP concentrations
  • No antibodies, coupling enzymes, wash steps or radioactivity
  • One substrate across 30 kinases simplifies selectivity and panel work
Learn about PhosphoSens® technology →

Frequently asked questions

Which kinases is AQT0734 validated for?

AQT0734 is validated for 30 kinases (1 wild-type and 29 mutant), including EGFR. See the full list above.

Which PhosphoSens assay formats use AQT0734?

AQT0734 is the PhosphoSens-Kinetic substrate for 30 kinases.

What sizes is AQT0734 available in?

1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.

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