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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About CAMK1G
CAMK1G (Calcium/Calmodulin-Dependent Protein Kinase IG) is a member of the CAMK1 subfamily of serine/threonine kinases, which are activated by the calcium-sensing protein calmodulin upon intracellular calcium elevation. Like other CAMK1 family members, CAMK1G requires binding of Ca2+/calmodulin to relieve autoinhibitory domain suppression and achieve full activation, with upstream CaMKK1/CaMKK2 providing additional activating phosphorylation on the activation loop. CAMK1G is expressed predominantly in neuronal tissues and has been implicated in neurite outgrowth, synaptic plasticity, and neuronal differentiation. Key substrates include CREB and synapsin family proteins involved in transcriptional regulation and vesicle trafficking. Pathway involvement spans CREB-mediated gene expression, MAPK/ERK signaling crosstalk, and mTORC1 regulation. Disease relevance includes neurological disorders, epilepsy, and emerging roles in certain cancers where calcium signaling is dysregulated. As a druggable kinase with a well-defined catalytic domain and disease-associated expression, CAMK1G represents a compelling target for CNS-focused drug discovery programs seeking selective CAMK1 subfamily inhibitors.
Why PhosphoSens for CAMK1G?
CAMK1G presents significant assay challenges: it requires Ca2+/calmodulin supplementation for activation, making traditional endpoint assays prone to variability from incomplete activation states and signal artifacts. ADP-Glo and HTRF endpoint formats cannot distinguish partial from full activation kinetics, miss burst phases, and are incompatible with covalent inhibitor characterization. Radiometric assays introduce safety burdens and cannot track real-time progress curves. PhosphoSens continuous fluorescent kinase assays overcome these limitations by monitoring substrate phosphorylation in real time under physiological ATP concentrations, generating full progress curves that capture enzyme activation dynamics, calmodulin concentration dependence, and inhibitor kinetics including covalent kinact/KI determination. This enables accurate IC50 measurements uncontaminated by endpoint timing artifacts, faithful Km/Vmax determination, and robust selectivity profiling essential for developing CNS-targeted CAMK1G inhibitors.
Key Pathways:
Ca2+/Calmodulin Signaling
CREB-Mediated Transcription
MAPK/ERK Signaling
mTORC1 Pathway
Talk to a Scientist
Have questions about CAMK1G assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time CAMK1G Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative CAMK1G activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0420
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0420)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0420
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CAMK1G?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF CAMK1G Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative CAMK1G activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0420
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0420)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0420
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CAMK1G?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteNo recombinant enzymes are currently available for CAMK1G.
Frequently Asked Questions: CAMK1G Assays
Does CAMK1G require calmodulin and calcium for activity in PhosphoSens assays?
Yes, CAMK1G requires supplementation with exogenous calmodulin and calcium chloride to achieve activation in biochemical assays. PhosphoSens assays are fully compatible with these cofactors, and the continuous real-time format allows direct visualization of how calmodulin concentration influences enzyme activation kinetics and substrate turnover rates.
Can PhosphoSens assays measure covalent or irreversible inhibitors of CAMK1G?
Yes, the continuous progress curve format of PhosphoSens technology is uniquely suited to characterize covalent inhibitors by enabling direct measurement of kinact and KI parameters from real-time fluorescence data. This is not possible with endpoint ADP-Glo or HTRF formats, which provide only a single time-point snapshot and can significantly misreport potency for time-dependent inhibitors.
What substrate is recommended for CAMK1G PhosphoSens assays?
AssayQuant's CSox-based peptide substrates optimized for CAMK1 family kinases are recommended for CAMK1G assays, providing high signal-to-background and sensitivity at physiological ATP concentrations. Running assays at physiological ATP avoids the artificial competitive advantage seen with ATP-depleting detection reagents, ensuring inhibitor IC50 values are translationally relevant.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ CAMK1G Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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