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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About AKT2
AKT2 (AKT Serine/Threonine Kinase 2) belongs to the AGC kinase superfamily and is one of three closely related AKT isoforms (AKT1, AKT2, AKT3). AKT2 is activated downstream of phosphoinositide 3-kinase (PI3K) through sequential phosphorylation at Thr309 by PDK1 and Ser474 by mTORC2, driving full catalytic activation. It preferentially phosphorylates substrates bearing the RxRxxS/T consensus motif, including GSK3β, FOXO1, AS160 (TBC1D4), and IRS1, coordinating insulin-mediated glucose uptake, glycogen synthesis, and GLUT4 translocation. AKT2 plays a dominant role in metabolic tissues — liver, skeletal muscle, and adipose — distinguishing it from the more proliferative roles of AKT1. Dysregulation through PIK3CA mutation, PTEN loss, or AKT2 amplification drives type 2 diabetes, insulin resistance, and cancers including breast, ovarian, pancreatic, and colorectal malignancies. Its central position in PI3K/AKT/mTOR signaling and metabolic control makes AKT2 a compelling and therapeutically validated drug target for both oncology and metabolic disease programs.
Why PhosphoSens for AKT2?
AKT2 drug discovery presents several assay challenges: the enzyme requires careful lipid cofactor considerations reflecting its PIP3-dependent activation, and its high homology with AKT1 and AKT3 demands isoform-selective profiling. Traditional endpoint assays like ADP-Glo and HTRF capture only a single time point, missing burst kinetics, substrate depletion artifacts, and non-linear progress curve behavior that can obscure true inhibitor mechanism. Radiometric assays introduce safety and throughput burdens. PhosphoSens continuous fluorescent kinase assays use CSox-based peptide substrates to monitor AKT2 substrate phosphorylation in real time at physiological ATP concentrations, generating complete progress curves that reveal mechanism of inhibition, enable accurate Ki determination, and faithfully capture covalent inhibitor kinact/KI parameters — critical for next-generation AKT2 covalent allosteric inhibitors. The homogeneous, no-wash format accelerates SAR campaigns and reduces false positives common in endpoint formats.
Key Pathways:
PI3K/AKT/mTOR signaling
Insulin signaling
FOXO transcription factor regulation
Glucose metabolism and GLUT4 trafficking
Approved Inhibitors:
Capivasertib (Truqap, HR+/HER2- breast cancer in combination with fulvestrant)
Talk to a Scientist
Have questions about AKT2 assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time AKT2 Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative AKT2 activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0536
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0536)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0536
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for AKT2?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF AKT2 Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative AKT2 activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0232
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0232)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0232
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for AKT2?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for AKT2
Wild-type and mutant forms of AKT2 for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
AKT2, Active Full Length
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-A17-10G
- Mutation: Full Length
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~85 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: AKT2 Assays
How does PhosphoSens measure AKT2 kinase activity in real time?
PhosphoSens uses proprietary CSox-containing peptide substrates that undergo a fluorescence change upon phosphorylation by AKT2, enabling continuous, real-time monitoring without antibodies or secondary detection reagents. This generates full kinetic progress curves from a single reaction well, allowing calculation of initial rates, Km, Vmax, and inhibitor IC50 values with high accuracy. The homogeneous format is compatible with standard fluorescence plate readers.
Can PhosphoSens AKT2 assays distinguish isoform-selective inhibitors from pan-AKT inhibitors?
Yes u2014 by running parallel continuous kinase assays with AKT1, AKT2, and AKT3 under matched conditions and physiological ATP concentrations, PhosphoSens enables direct isoform selectivity profiling in the same assay format. Real-time progress curves reveal differences in potency and inhibition kinetics across isoforms that single-timepoint ADP-Glo or HTRF assays may obscure. This is particularly valuable for programs targeting AKT2 selectively for metabolic indications.
Is the PhosphoSens AKT2 assay suitable for characterizing covalent allosteric inhibitors?
Absolutely u2014 PhosphoSens is uniquely suited for covalent inhibitor characterization because continuous progress curves directly reveal time-dependent inhibition, enabling determination of kinact and KI using established kinetic models. Unlike endpoint assays that miss the temporal dimension of covalent engagement, PhosphoSens captures the full inhibition trajectory in real time. This is especially relevant for AKT2 covalent allosteric inhibitors that target the PH domain-DFG-out conformation.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ AKT2 Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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