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About ABL1
ABL1 (ABL proto-oncogene 1) is a non-receptor tyrosine kinase belonging to the Abl family, which includes ABL1 and ABL2 (ARG). Under normal physiological conditions, ABL1 activity is tightly regulated through intramolecular autoinhibition, with the SH3 and SH2 domains clamping the kinase domain in an inactive conformation. Activation occurs via displacement of this autoinhibitory clamp by upstream signals, scaffolding proteins, or oncogenic rearrangements. Key substrates include CRKL, STAT5, FAK, and the adaptor protein DOK1, linking ABL1 to cell proliferation, survival, cytoskeletal remodeling, and DNA damage response. In chronic myelogenous leukemia (CML) and a subset of acute lymphoblastic leukemias (ALL), the chromosomal translocation t(9;22) generates the BCR-ABL1 fusion oncoprotein, constitutively activating kinase activity and driving uncontrolled myeloid expansion. This makes ABL1 one of the most validated oncology drug targets in history, and the success of imatinib as a molecularly targeted therapy established the proof-of-concept for kinase inhibitor drug discovery across oncology.
Why PhosphoSens for ABL1?
Profiling ABL1 inhibitors presents several assay challenges: the kinase is frequently studied at physiological ATP concentrations to accurately capture competitive inhibitor potency, and the BCR-ABL1 fusion introduces complex regulatory dynamics that can confound endpoint measurements. Traditional ADP-Glo and HTRF assays require incubation stop points that miss transient kinetic phenomena such as slow-on/slow-off binding, covalent inactivation (kinact/KI), and induced-fit mechanisms critical for third-generation inhibitors like ponatinib and asciminib. Radiometric formats add safety and throughput limitations. AssayQuant's PhosphoSens continuous fluorescent assay monitors substrate phosphorylation in real time using physiological ATP, generating full progress curves that capture time-dependent inhibition, covalent kinact/KI parameters, and inhibitor mechanism of action without the endpoint artifacts that can misclassify allosteric STAMP inhibitors like asciminib.
Key Pathways:
BCR-ABL1 Signaling
RAS/MAPK Pathway
PI3K/AKT/mTOR Pathway
DNA Damage Response
Approved Inhibitors:
Imatinib (Gleevec, CML/ALL/GIST
Dasatinib (Sprycel, CML/ALL
Nilotinib (Tasigna, CML
Bosutinib (Bosulif, CML
Ponatinib (Iclusig, CML/ALL
Asciminib (Scemblix, CML
Bafetinib (not FDA-approved)
Talk to a Scientist
Have questions about ABL1 assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time ABL1 Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative ABL1 activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0032
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0032)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0032
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
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Request a QuoteNo PhosphoSens-Red format is currently available for ABL1.
Ready to request pricing for ABL1?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for ABL1
Wild-type and mutant forms of ABL1 for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
ABL1, Active
Mouse • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-A03-11H
- Species: Mouse
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300mM NaCl, 50-200mM imidazole, 0-0.1mM PMSF, 0.2-1.0mM DTT, 10-25% glycerol
- Molecular Weight: ~135 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: ABL1 Assays
Why is it important to use physiological ATP concentrations when profiling ABL1 inhibitors?
Most cellular ATP concentrations range from 1 to 5 mM, and competitive inhibitor IC50 values are highly sensitive to ATP concentration due to the Cheng-Prusoff relationship. Assays run at artificially low ATP levels can dramatically underestimate inhibitor potency relative to the cellular environment. AssayQuant PhosphoSens assays are validated at physiological ATP concentrations, providing IC50 and Ki values with direct translational relevance.
Can the PhosphoSens assay distinguish allosteric ABL1 inhibitors like asciminib from ATP-competitive inhibitors?
Yes. Because PhosphoSens generates continuous real-time progress curves, the kinetic signatures of allosteric inhibitors targeting the ABL1 myristoyl-binding pocket differ measurably from ATP-competitive mechanisms, enabling mechanistic classification. This is particularly valuable for characterizing STAMP inhibitors and combination inhibitor strategies where distinguishing mechanism of action is critical for lead optimization.
How does the PhosphoSens platform handle covalent or time-dependent ABL1 inhibitor characterization?
PhosphoSens continuously monitors phosphorylation over time, allowing direct observation of the time-dependent loss of enzyme activity characteristic of covalent or slow-binding inhibitors. From these progress curves, kinact and KI parameters can be extracted, which are not accessible from single-timepoint endpoint assays such as ADP-Glo or HTRF, making PhosphoSens essential for covalent ABL1 inhibitor programs.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ ABL1 Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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