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PhosphoSens EGFR [T790M] Protein Kinase Assays, Substrates & Recombinant Enzymes
Also known as: EGFR • Epidermal growth factor receptor • erb-b2 receptor tyrosine kinase 1 • ERBB • ERBB1 • mENA • HER1 • erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian) • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog • Receptor tyrosine-protein kinase ErbB-1 • V-erb-b oncogene homologue
![PhosphoSens EGFR [T790M] kinase assay kit — continuous real-time fluorescence](https://www.assayquant.com/hs-fs/hubfs/PhosphoSens%20Packaging%20C%20(3).jpg?width=450&name=PhosphoSens%20Packaging%20C%20(3).jpg)
EGFR [T790M] at a glance. EGFR [T790M] activity is measured continuously and directly with the PhosphoSens® AQT0734 substrate, with no antibodies or coupling enzymes.
- Assay formats
- Kinetic (continuous)
- Substrate
- AQT0734
- Assay kits
- 200 or 1,000 assays, from $425
- Publications
- 27 peer-reviewed studies
About EGFR [T790M]
EGFR (Epidermal Growth Factor Receptor, ErbB1/HER1) is a receptor tyrosine kinase belonging to the ErbB family, which also includes HER2, HER3, and HER4. Upon binding EGF or related ligands, EGFR undergoes homo- or heterodimerization, triggering autophosphorylation at intracellular tyrosine residues including Y1068, Y1086, Y1148, and Y1173. These phosphorylation events recruit adaptor proteins such as GRB2 and SHC, activating downstream signaling through RAS-MAPK, PI3K-AKT-mTOR, and STAT pathways to drive cell proliferation, survival, and differentiation. Key substrates include PLCgamma, GAB1, and the receptor itself. EGFR is overexpressed or mutated in numerous cancers, with activating mutations in exons 18-21 (notably L858R and exon 19 deletions) conferring constitutive kinase activity. EGFR is one of the most validated oncology drug targets, with multiple approved inhibitor generations targeting the ATP-binding site or covalently modifying C797, making it a central focus of kinase drug discovery programs.
Why PhosphoSens for EGFR [T790M]?
EGFR drug discovery presents distinct assay challenges including the need to accurately characterize covalent inhibitors such as osimertinib and afatinib, where traditional endpoint assays like ADP-Glo and HTRF fail to capture the time-dependent kinact/KI parameters essential for covalent mechanism characterization. Radiometric assays offer sensitivity but generate radioactive waste and provide only single-timepoint snapshots. PhosphoSens continuous fluorescent kinase assays monitor EGFR substrate phosphorylation in real time using physiological ATP concentrations, generating complete progress curves that directly reveal covalent inhibitor kinetics, competitive binding modes, and subtle mechanistic differences between first-, second-, and third-generation inhibitors. This real-time approach eliminates endpoint artifacts from product inhibition or enzyme instability, enables accurate IC50 and Ki determination under near-physiological conditions, and is readily adaptable for high-throughput profiling of mutant EGFR variants including L858R, T790M, and C797S resistance mutations.
Key Pathways:
test
Approved Inhibitors:
Gefitinib (Iressa, NSCLC
Erlotinib (Tarceva, NSCLC/Pancreatic Cancer
Afatinib (Gilotrif, NSCLC
Osimertinib (Tagrisso, NSCLC
Lapatinib (Tykerb, HER2+ Breast Cancer
Neratinib (Nerlynx, HER2+ Breast Cancer
Dacomitinib (Vizimpro, NSCLC
Vandetanib (Caprelsa, Medullary Thyroid Cancer
Tucatinib (Tukysa, HER2+ Breast Cancer)
Talk to a Scientist
Have questions about EGFR [T790M] assay design, selectivity panels, or covalent inhibitor characterization?
Ask Our Scientists →
How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time EGFR [T790M] Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative EGFR [T790M] activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0734
Substrate: AQT0734
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0734)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0734
See every target AQT0734 assays →
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
- Catalog #: AQT0734
- Phospho-acceptor: Tyrosine
- Validated for: 30 kinases
- Formats: PhosphoSens-Kinetic
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
One substrate, many targets
AQT0734 also assays PDGFRA (3 mutants), plus 26 other EGFR variants
Validated with the same PhosphoSens® substrate, so you can run a selectivity panel in one assay format.
Ready to request pricing for EGFR [T790M]?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
No PhosphoSens-Red format is currently available for EGFR [T790M].
Ready to request pricing for EGFR [T790M]?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
No recombinant enzymes are currently available for EGFR [T790M].
Published research on EGFR
Peer-reviewed studies that used AssayQuant assays, substrates or services for EGFR.
Enozertinib Is a Selective, Brain-Penetrant EGFR Inhibitor for Treating Non-Small Cell Lung Cancers with EGFR Exon 20 and Atypical Mutations
Junttila MR, Repellin CE, Salaniwal S, … Friedman LS
Structural Studies of Fourth-Generation EGFR Inhibitors Reveal Insights into Selective T790M and C797S Targeting
Damghani T, Song S, Lin KS, … Heppner DE
TAS3351 is a brain penetrable EGFR-TKI that overcomes T790M and C797S resistant mutations
Kasuga H, Kataoka Y, Yamamoto F, … Mizuarai S
EGFR biochemical kinetic parameters determined with the PhosphoSens Kinase Assay
Covalent Alkynylpyridopyrimidinones Targeting Cysteine 775 of the Epidermal Growth Factor Receptor Overcome Resistance to Current Therapies
Stewart HL, Bordoni C, Jennings CE, … Waring MJ
Kinetic data for covalent C775-targeting EGFR inhibitors determined by AssayQuant
Discovery of STX-721, a Covalent, Potent, and Highly Mutant-Selective EGFR/HER2 Exon20 Insertion Inhibitor for the Treatment of Non-Small Cell Lung Cancer
Milgram BC, Borrelli DR, Brooijmans N, … Guzman-Perez A
KI/kinact determined by ChEF assay with AQT0001 substrate
FL30: an epidermal growth factor kinase inhibitor overcoming T790M and C797S mutations through unique conformational modulation mechanism
Romagnoli E, Laudadio E, Mobbili G, … et al.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ EGFR [T790M] Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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