AQT0426 PhosphoSens® Serine/threonine Kinase Substrate

A PhosphoSens® serine/threonine kinase substrate validated for 26 kinases (25 wild-type, 1 mutant), including CAMK1, CAMK4, DCLK1. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.

Catalog number
AQT0426
Enzyme class
Kinase
Residue specificity
Serine/threonine
Validated kinases
26
Assay formats
Kinetic · 26
Kinase families
7

Kinases validated with AQT0426

Every kinase below has a validated PhosphoSens® assay using AQT0426. Select a target to see its kits, recombinant enzyme, pricing and protocols.

KinaseTypeFamilyFormat with AQT0426Link
CAMK1 Wild-type CAMK1 Kinetic View assay →
CAMK4 Wild-type CAMK1 Kinetic View assay →
DCLK1 Wild-type DCAMKL Kinetic View assay →
DCLK2 Wild-type DCAMKL Kinetic View assay →
MAPKAPK2 Wild-type MAPKAPK Kinetic View assay →
MAPKAPK5 Wild-type MAPKAPK Kinetic View assay →
MELK Wild-type CAMKL Kinetic View assay →
MELK [T460M] Mutant CAMKL Kinetic View assay →
PHKG1 Wild-type PHK Kinetic View assay →
PHKG2 Wild-type PHK Kinetic View assay →
PKN2 Wild-type PKN Kinetic View assay →
PRKAA1-B1-G1 Wild-type CAMKL Kinetic View assay →
PRKAA1-B1-G2 Wild-type CAMKL Kinetic View assay →
PRKAA1-B1-G3 Wild-type CAMKL Kinetic View assay →
PRKAA1-B2-G1 Wild-type CAMKL Kinetic View assay →
PRKAA1-B2-G2 Wild-type CAMKL Kinetic View assay →
PRKAA1-B2-G3 Wild-type CAMKL Kinetic View assay →
PRKAA2-B1-G1 Wild-type CAMKL Kinetic View assay →
PRKAA2-B1-G2 Wild-type CAMKL Kinetic View assay →
PRKAA2-B1-G3 Wild-type CAMKL Kinetic View assay →
PRKAA2-B2-G1 Wild-type CAMKL Kinetic View assay →
PRKAA2-B2-G2 Wild-type CAMKL Kinetic View assay →
PRKAA2-B2-G3 Wild-type CAMKL Kinetic View assay →
SNRK Wild-type CAMKL Kinetic View assay →
TSSK2 Wild-type TSSK Kinetic View assay →
TSSK3 Wild-type TSSK Kinetic View assay →

Bulk substrate

AQT0426 PhosphoSens® Kinase Substrate

Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0426 are on each target's assay page.

Price: $2795 USD

How PhosphoSens® substrates work

PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.

  • Real-time progress curves, not single end-points
  • Run at physiologically relevant ATP concentrations
  • No antibodies, coupling enzymes, wash steps or radioactivity
  • One substrate across 26 kinases simplifies selectivity and panel work
Learn about PhosphoSens® technology →

Frequently asked questions

Which kinases is AQT0426 validated for?

AQT0426 is validated for 26 kinases (25 wild-type and 1 mutant), including CAMK1, CAMK4, DCLK1. See the full list above.

Which PhosphoSens assay formats use AQT0426?

AQT0426 is the PhosphoSens-Kinetic substrate for 26 kinases.

What sizes is AQT0426 available in?

1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.

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