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PhosphoSens CDK17/CycY Protein Kinase Assays, Substrates & Recombinant Enzymes
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About CDK17/CycY
CDK17 (cyclin-dependent kinase 17), also known as PCTK3, belongs to the PCTAIRE subfamily of CDKs, a group of atypical cyclin-dependent kinases characterized by replacement of the canonical PSTAIRE helix motif. CDK17 associates with cyclin Y (CycY) to form an active complex, and activation is further regulated by phosphorylation within the T-loop by upstream kinases. Unlike canonical cell-cycle CDKs, CDK17/CycY is implicated in neuronal differentiation, vesicle trafficking, and Wnt signaling through phosphorylation of substrates including p27Kip1, PP2A regulatory subunits, and components of the endocytic machinery. The kinase is expressed predominantly in brain and testis, implicating it in post-mitotic neuronal function. Emerging evidence links CDK17 overexpression or amplification to glioblastoma, breast cancer, and other solid tumors, where it may promote cell survival and chemoresistance. Its restricted tissue expression and roles in oncogenic signaling make CDK17 an attractive target for selective oncology drug discovery programs.
Why PhosphoSens for CDK17/CycY?
CDK17/CycY presents significant assay challenges: it requires co-expression of cyclin Y for catalytic activity, displays low intrinsic activity compared to canonical CDKs, and operates best at physiological ATP concentrations where endpoint luminescence assays such as ADP-Glo suffer from poor signal-to-noise at low substrate turnover. HTRF and radiometric formats require fixed timepoints that can miss burst kinetics or substrate depletion artifacts, and antibody-based readouts risk cross-reactivity with related PCTAIRE family members. PhosphoSens continuous fluorescent assays use chelation-enhanced fluorescent peptide substrates to generate real-time progress curves at physiological ATP, enabling accurate Km(ATP) determination critical for selectivity profiling against the CDK family. Continuous kinetics readily capture slow-binding or covalent inhibitor behavior through kinact/KI measurements, and eliminates endpoint variability that confounds low-activity kinase characterization.
Key Pathways:
Wnt/beta-catenin signaling
Vesicle trafficking and endocytosis
Neuronal differentiation
Cell cycle regulation
Talk to a Scientist
Have questions about CDK17/CycY assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time CDK17/CycY Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative CDK17/CycY activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0730
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0730)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0730
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CDK17/CycY?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF CDK17/CycY Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative CDK17/CycY activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0730
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0730)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0730
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CDK17/CycY?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteNo recombinant enzymes are currently available for CDK17/CycY.
Frequently Asked Questions: CDK17/CycY Assays
What substrate does AssayQuant recommend for CDK17/CycY kinase assays?
AssayQuant offers PhosphoSens CSox-based peptide substrates optimized for CDK family members, including substrates derived from known CDK17 phosphorylation motifs. These substrates generate a real-time fluorescent signal upon phosphorylation, enabling continuous monitoring without antibodies or secondary detection reagents. Contact our assay development team for substrate selection guidance specific to CDK17.
Why is physiological ATP concentration important when profiling CDK17 inhibitors?
CDK17 inhibitors are typically screened as part of broader CDK selectivity panels, and many competitive inhibitors show strong ATP-dependence in their apparent potency. Running assays at physiological ATP (1-5 mM) using PhosphoSens technology ensures that IC50 values accurately reflect cellular inhibitory potential, preventing false selectivity conclusions that arise from low-ATP endpoint assays like ADP-Glo.
Can PhosphoSens assays detect covalent or slow-binding inhibitors of CDK17?
Yes. Because PhosphoSens generates continuous real-time progress curves, time-dependent inhibition, slow-binding kinetics, and covalent inactivation are directly visible as curvature or progressive inhibition in the fluorescence signal over time. This allows determination of kinact and KI parameters essential for characterizing irreversible or pseudo-irreversible CDK17 inhibitor candidates.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ CDK17/CycY Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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