Activity-Based Biochemical Kinase Panel Screening and Profiling Services
AssayQuant's KinSight™ service helps guide improved drug discovery and development with kinetic insights into kinome-wide selectivity and mechanism of action (MOA) using our unique continuous assay format. Make better-informed decisions about therapeutic opportunities and potential off-target liabilities with high-confidence.
Go Beyond % Inhibition: Get Actionable Kinetic Insights with KinSight Kinase Profiling
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True Kinetic Understanding: Directly measure and quantify activity and inhibition in real-time - no endpoint assumptions - for accurate potency, selectivity, and MOA insights.
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Variable ATP Concentrations for Deeper Insights: Profile compounds at ATP Km to maximize potency data, or at 1 mM ATP for physiological relevance.
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Actionable Reports, Fast: Receive comprehensive reports with expert annotations highlighting exploitable kinetics (like TDI) – all within two weeks.
KinSight™ Quantitative Kinome Profiling Services
Kinase Panel Screening Details
Testing Performed: Selectivity profiling against 400 unique kinase targets run at single compound concentration, in duplicate, at 1mM ATP or each targets ATP Km.
Deliverables: Detailed Kinetic Report for each compound that includes annotated progress curves to call out exploitable mechanisms of actions (MOA) and % inhibition presented in:
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Numerical Format (Tabular), % inhibition for each enzyme
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Graphical Format (Waterfall Plot), % inhibition for each enzyme
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Graphical Format (Waterfall Plot), >70% inhibition
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Visual Format (Kinome Tree), >70% inhibition
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Fast Turnaround Time and Expert Guidance: Screening complete in two weeks so you get results and important functional information about the enzymology of the kinases and nature of the compound quickly!
Customize Your Kinase or Phosphatase Panel Today
In addition to our broad kinome selectivity panel, we can create custom kinase panels, add mutants to our full panel and performing selectivity screening across our panel of 35+ phosphatases.
The KinSight Advantage: Real-Time Kinetic Data
Providing a Progress Curve in Every Well
AssayQuant's KinSight™ profiling services leverage our unique PhosphoSens® Technology, a continuous assay format that directly measures substrate phosphorylation throughout the entire reaction. Instead of relying on single-point estimates, this approach generates a full progress curve in every well, yielding the actual reaction rate from dozens of data points.
Understanding the true reaction rate provides higher confidence data, free from the assumptions inherent in endpoint assays. This makes KinSight™ profiling valuable at all stages of preclinical drug discovery, helping identify compounds with poor selectivity, pinpoint optimization opportunities, or confirm off-target interactions early.
Detailed Reports by our Team of Expert Enzymologists
Our scientists utilize the rich kinetic data from the continuous assay to generate detailed reports for each compound. These reports go beyond simple inhibition values, providing functional insights into kinase enzymology and compound behavior to guide your development decisions.
Our combination of an industry-leading panel, validated automation, and in-depth kinetic analysis delivers exceptional kinome profiling results. You can swiftly determine kinome-wide selectivity, gaining crucial insights into MOA and relative potency directly from the profiling data.
Continuous (Kinetic, Real-Time) Activity Monitoring
Capture true inhibition kinetics without linearity assumptions for higher accuracy and deeper understanding.
Versatile ATP Concentrations
Profile at ATP Km for potency or 1 mM ATP for physiological relevance—or both for maximal MOA insights.
Informative Kinetic Profiling Report
Go beyond % inhibition with annotated kinetic data, revealing exploitable MOA insights.
Industry Leading Kinase Panel
Access 400+ quality-tested wild-type kinases, plus mutant, cascade, and custom panel options.
Correlates With Cell-Based Assays
Assay conditions compatible with physiological cofactors mimic cellular environments.
Fast Turnaround Time and Expert Guidance
Get comprehensive screening data and expert functional insights in just two weeks.
Resources
AssayQuant's Kinome Profiling Services Are More Than Just an Alternative—Access A Better Approach to Kinome Profiling Today
Most biochemical kinase profiling services rely on endpoint assays, providing only a single snapshot of enzyme activity. But kinase inhibition is dynamic—why settle for incomplete data?
AssayQuant's KinSight Kinase Panel Screening and Profiling Services deliver everything you need—and more—with a next-generation approach to kinase inhibitor characterization.
With AssayQuant's KinSight profiling platform, you don’t just get data—you get kinetic insights into selectivity and mechanism of action (MOA) for your drug discovery research.
Kinome Tree Mapping Application
Visualize your kinome profiling data with customizable kinome trees.
Kinome Profiling With a Continuous Assay
Explore an Example Data Report
Interested in exploring our full data report? Download a sample data report complete with known inhibitors and reference compounds. We tested them at variable compound concentrations with ATP concentrations at either Km or 1 mM for each of our 400+ kinases! Preview the possibilities of kinetic insights into selectivity and mechanism of action (MOA) for your drug discovery research.
Contact our team and let us build the ideal panel for your profiling needs.
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