Kinome Profiling Services Compared: KinSight vs Eurofins, Reaction Biology, Thermo Fisher and Carna
Most kinase profiling services read each well once, or measure binding instead of activity. KinSight™ measures kinase activity continuously, so every well returns a full progress curve that shows time-dependent inhibition, lag phases and assay artifacts a single reading can miss.
Three questions that separate profiling services
1. Binding or activity? Binding panels such as KINOMEscan® report how tightly a compound binds a kinase, not whether it blocks phosphorylation. Activity panels measure inhibition directly.
2. One reading or a progress curve? Radiometric, ADP-Glo™, Z'-LYTE™ and mobility-shift screens typically report one value per well; some providers offer time-course reads on request. PhosphoSens® records the whole reaction in every well of the standard screen.
3. Kinases only, or phosphatases too? KinSight profiles 560+ kinases and 50 phosphatases with the same continuous readout and peptide substrates. See phosphatase profiling.
How the main providers compare
What each provider states on its own public service pages, as of October 2026. Panel sizes and formats change, so check with each provider for current details.
| Provider | Kinase profiling format | Kinase panel (as stated) | Readout per well | Phosphatase profiling |
|---|---|---|---|---|
| AssayQuant KinSight™ | Activity: PhosphoSens® continuous fluorescence (direct, Sox-based) | 560+ kinases | Full progress curve in every well | 50 phosphatases, continuous readout, peptide substrates |
| Eurofins Discovery | Activity (KinaseProfiler™) and binding (KINOMEscan®) | 422 activity assays; 490+ binding assays | One activity or binding value per well | Not listed in its kinase services guide |
| Reaction Biology | Activity: radiometric ³³P (HotSpot™, 33PanQinase™) and ADP-Glo™ | 870+ kinase assays | Endpoint; time-course reads and pre-incubation for slow binders on request | 22 phosphatase assays using a small-molecule fluorogenic substrate (DiFMUP) |
| Thermo Fisher SelectScreen™ | Activity (Z'-LYTE™, Adapta™) and binding (LanthaScreen™ Eu) | 465+ kinase assays | Endpoint | Not listed on its kinase profiling page |
| Carna Biosciences | Activity: mobility shift assay, IMAP™ and ADP-Glo™ | 329 kinase targets | Endpoint | Not listed on its profiling page |
Sources: Eurofins Discovery kinase solution guide; Reaction Biology kinase screening brochure (Aug 2026); Reaction Biology phosphatase assays; Thermo Fisher SelectScreen kinase profiling; Carna Biosciences profiling service. Trademarks belong to their respective owners.
Advantages of Profiling With a Continuous Assay Format
Most kinome profiling platforms use endpoint assays—single time-point measurements that assume linear reaction kinetics. But this assumption often breaks down due to factors like lag phases, substrate depletion, enzyme instability, or time-dependent inhibition (TDI).
KinSight uses a continuous assay format to overcome these limitations. By monitoring enzyme activity in real time, we calculate actual reaction rates from multiple data points within the true linear range. This delivers higher-confidence selectivity and MOA insights, and enables direct detection of kinetic phenomena like TDI.
Continuous (Kinetic) Activity Monitoring
Capture true inhibition kinetics for higher accuracy and deeper understanding.
Versatile ATP Concentrations
Profile at ATP Km to maximize potency, 1 mM for physiological relevance—or both for maximal MOA insights.
Informative Kinetic Profiling Report
Go beyond % inhibition with annotated kinetic data, revealing exploitable MOA insights.
Industry Leading Kinase Panel
Access 560+ quality-tested kinases, plus mutant, cascade, and custom panel options.
Correlates With Cell-Based Assays
Our technology is compatible with physiological levels of ATP, Mg2+, Mn2+, and Ca2+ and incorporates relevant activators and cofactors.
Fast Turnaround Time and Expert Guidance
Get comprehensive screening data and expert functional insights in just two weeks.
"AssayQuant's PhosphoSens technology is uniquely suited to advance Vibliome's kinase inhibitor program. PhosphoSens provides a deep understanding of our compounds including specificity, potency, mode-of-inhibition, reversibility, and time-dependent inhibition. This, along with AssayQuant's world-class scientific support and fast turnaround times powered by automation, will make this a highly productive partnership that will accelerate Vibliome's drug development programs."
Kinase Panel Screening Details
Testing Performed:
Selectivity profiling against 560+ kinase targets run in kinetic mode at a single compound concentration, in duplicate, at 1mM ATP or each targets ATP Km.
Deliverables:
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Numerical Format (Tabular), % inhibition for each enzyme
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Graphical Format (Waterfall Plot), % inhibition for each enzyme
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Graphical Format (Waterfall Plot), >70% inhibition
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Visual Format (Kinome Tree), >70% inhibition
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Also includes annotated progress curves to call out exploitable mechanisms of actions (MOA).
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Fast Turnaround and Expert Guidance:
Screening complete in two weeks so you get results and important functional information about the enzymology of the kinases and nature of the compound quickly!
See the Rich Kinetic Data You'll Receive - Download Sample Report
Interested in exploring our full data report? Download a sample data report complete with known inhibitors and reference compounds. We tested them at variable compound concentrations with ATP concentrations at either Km or 1 mM for each of our 400+ wild-type kinases! Preview the possibilities of kinetic insights into selectivity and mechanism of action (MOA) for your drug discovery research.
We Also Offer Selectivity Profiling For Phosphatase Drug Discovery and Development!
We have the largest number of assays and use sensor phospho-peptide substrates to monitor activity kinetically (in real-time). Learn more by accessing out poster "Sox-based Sensor Phosphopeptides for Continuous, Homogeneous, and Quantitative Monitoring of Protein Phosphatase Activity" below:
How is KinSight different from Eurofins or Reaction Biology kinase profiling?
KinSight measures kinase activity continuously with PhosphoSens®, so each well gives a progress curve rather than a single value. Eurofins Discovery offers activity (KinaseProfiler) and binding (KINOMEscan) panels, and Reaction Biology runs radiometric activity assays. KinSight also profiles 50 phosphatases with the same readout.
What is the difference between binding and activity-based kinase profiling?
Binding profiling measures how tightly a compound binds a kinase. Activity profiling measures whether it actually inhibits phosphorylation. The two can rank compounds differently, so activity data is the more direct measure of functional selectivity.
Can KinSight detect time-dependent and covalent inhibitors in the primary screen?
Yes. Because every well is read continuously, curvature in the progress curve flags time-dependent inhibition in the screen itself. Hits can then be characterized with kinact/KI and residence-time studies through our time-dependent inhibition service.
Does AssayQuant profile phosphatases?
Yes. KinSight phosphatase profiling covers 50 phosphatases using physiological peptide substrates and the same continuous readout as the kinase panel.
Which ATP concentrations can I profile at?
KinSight kinase profiling runs at each kinase's ATP Km or at 1 mM ATP, or both, so you can compare potency at biochemical and physiological ATP.
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