Get the full 45-minute session
Standard IC₅₀ assays can seriously mislead when a compound shows time-dependent inhibition — and that's common for covalent kinase inhibitors and slow-binding reversible ones alike. This session walks through a proven three-step kinetic workflow for getting it right, demonstrated on five real EGFR inhibitors spanning fast-reversible to fully covalent mechanisms, with a close look at how to correctly determine kinact/KI, distinguish reversible from irreversible binding without rebinding artifacts, and interpret kinome-wide selectivity data for covalent compounds.
