AQT0420 PhosphoSens® Serine/threonine Kinase Substrate

A PhosphoSens® serine/threonine kinase substrate validated for 16 kinases, including CAMK1D, CAMK1G, CAMK4. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.

Catalog number
AQT0420
Enzyme class
Kinase
Residue specificity
Serine/threonine
Validated kinases
16
Assay formats
Kinetic · 3 Red · 16
Kinase families
2

Kinases validated with AQT0420

Every kinase below has a validated PhosphoSens® assay using AQT0420. Select a target to see its kits, recombinant enzyme, pricing and protocols.

KinaseTypeFamilyFormat with AQT0420Link
CAMK1D Wild-type CAMK1 KineticRed View assay →
CAMK1G Wild-type CAMK1 KineticRed View assay →
CAMK4 Wild-type CAMK1 Red View assay →
PNCK Wild-type CAMK1 KineticRed View assay →
PRKAA1-B1-G1 Wild-type CAMKL Red View assay →
PRKAA1-B1-G2 Wild-type CAMKL Red View assay →
PRKAA1-B1-G3 Wild-type CAMKL Red View assay →
PRKAA1-B2-G1 Wild-type CAMKL Red View assay →
PRKAA1-B2-G2 Wild-type CAMKL Red View assay →
PRKAA1-B2-G3 Wild-type CAMKL Red View assay →
PRKAA2-B1-G1 Wild-type CAMKL Red View assay →
PRKAA2-B1-G2 Wild-type CAMKL Red View assay →
PRKAA2-B1-G3 Wild-type CAMKL Red View assay →
PRKAA2-B2-G1 Wild-type CAMKL Red View assay →
PRKAA2-B2-G2 Wild-type CAMKL Red View assay →
PRKAA2-B2-G3 Wild-type CAMKL Red View assay →

Bulk substrate

AQT0420 PhosphoSens® Kinase Substrate

Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0420 are on each target's assay page.

Price: $2795 USD

How PhosphoSens® substrates work

PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.

  • Real-time progress curves, not single end-points
  • Run at physiologically relevant ATP concentrations
  • No antibodies, coupling enzymes, wash steps or radioactivity
  • One substrate across 16 kinases simplifies selectivity and panel work
Learn about PhosphoSens® technology →

Frequently asked questions

Which kinases is AQT0420 validated for?

AQT0420 is validated for 16 kinases, including CAMK1D, CAMK1G, CAMK4. See the full list above.

Which PhosphoSens assay formats use AQT0420?

AQT0420 is the PhosphoSens-Kinetic substrate for 3 kinases. It is the PhosphoSens-Red substrate for 16 kinases.

What sizes is AQT0420 available in?

1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.

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