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PhosphoSens CDK14/CycY Protein Kinase Assays, Substrates & Recombinant Enzymes
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About CDK14/CycY
CDK14 (cyclin-dependent kinase 14, also known as PFTK1) is an atypical member of the CDK family that associates with cyclin Y (CycY) and cyclin Y-like 1 to form active complexes. Unlike canonical cell-cycle CDKs, CDK14/CycY is regulated in part through membrane recruitment and Wnt ligand stimulation, positioning it as a critical node linking extracellular signals to intracellular phosphorylation cascades. Key substrates include LRP6, a Wnt co-receptor whose phosphorylation primes it for signaling, and components of the cell-cycle machinery including CDK1. CDK14 participates in G2/M progression and has been implicated in neuronal differentiation and spermatogenesis. Dysregulation of CDK14 occurs in multiple cancers, including hepatocellular carcinoma, breast cancer, and gastric cancer, where overexpression correlates with poor prognosis. Its role in amplifying Wnt/beta-catenin signaling makes CDK14 an attractive drug target, particularly in Wnt-driven malignancies where upstream pathway components are difficult to inhibit selectively.
Why PhosphoSens for CDK14/CycY?
CDK14/CycY presents distinct assay challenges: its activity is relatively low compared to canonical CDKs, and it requires co-expression of cyclin Y for robust activation, making endpoint assays susceptible to variability from incomplete complex formation. ADP-Glo and HTRF approaches capture only a single time point, masking nonlinear kinetics caused by substrate depletion or inhibitor time-dependence — critical liabilities when characterizing covalent or slow-binding inhibitors. Radiometric assays add handling complexity and waste disposal burdens. PhosphoSens continuous fluorescent kinase assays use a chelation-enhanced fluorescent (CHEF) peptide substrate to monitor CDK14-mediated phosphorylation in real time at physiological ATP concentrations, generating full progress curves. This enables accurate Km and Vmax determination, direct detection of kinact/KI for covalent inhibitors, and identification of time-dependent inhibition without endpoint artifacts, dramatically improving hit quality and mechanistic resolution.
Key Pathways:
Wnt/beta-catenin signaling
Cell cycle G2/M regulation
LRP6 receptor phosphorylation
Neuronal differentiation signaling
Talk to a Scientist
Have questions about CDK14/CycY assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time CDK14/CycY Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative CDK14/CycY activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0730
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0730)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0730
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CDK14/CycY?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF CDK14/CycY Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative CDK14/CycY activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0730
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0730)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0730
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for CDK14/CycY?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteNo recombinant enzymes are currently available for CDK14/CycY.
Frequently Asked Questions: CDK14/CycY Assays
What substrate does AssayQuant use for CDK14/CycY activity assays?
AssayQuant offers a CHEF-peptide substrate engineered to report CDK14-mediated phosphorylation via real-time fluorescence changes. The substrate sequence is optimized for CDK14/CycY selectivity and is compatible with physiological ATP concentrations, enabling accurate kinetic measurements without the interference seen in coupled or antibody-based endpoint formats.
How does the PhosphoSens assay handle the low intrinsic activity of CDK14/CycY?
PhosphoSens continuous kinetics are highly sensitive because fluorescence is monitored across the full reaction time course, allowing signal accumulation that compensates for low turnover rates. This avoids the signal-to-noise limitations of single-endpoint formats, which often fail to detect weak kinase activity reliably, and permits accurate progress curve analysis even at low enzyme concentrations.
Can the PhosphoSens CDK14 assay be used to characterize covalent inhibitors?
Yes. Because PhosphoSens generates continuous progress curves at physiological ATP, it directly captures time-dependent inhibition profiles required to calculate kinact and KI for covalent inhibitors. This is not achievable with ADP-Glo or HTRF endpoint assays, making PhosphoSens the preferred format for covalent CDK14 inhibitor campaigns targeting the ATP-binding cysteine or allosteric sites.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ CDK14/CycY Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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