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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About BRSK1
BRSK1 (Brain-Specific Serine/Threonine Kinase 1), also known as SAD-B, belongs to the CAMK-like (CAMKL) superfamily and is a close relative of AMPK and MARK kinases. BRSK1 is activated by LKB1-mediated phosphorylation at Thr189 within its activation loop, placing it downstream of the master energy-sensing LKB1-AMPK axis. Key substrates include tau protein, CDC25 phosphatases, and components of the cell polarity machinery. BRSK1 plays a central role in neuronal polarity establishment, axon differentiation, and synaptic vesicle trafficking. Beyond the central nervous system, BRSK1 participates in cell cycle checkpoint regulation and DNA damage responses. Dysregulation of BRSK1 has been implicated in Alzheimer's disease through aberrant tau hyperphosphorylation, as well as in various cancers where LKB1 pathway mutations are prevalent. Its selective expression pattern and participation in LKB1-driven tumor suppressor networks make BRSK1 an emerging drug target for neurodegenerative disease and oncology.
Why PhosphoSens for BRSK1?
BRSK1 presents several assay challenges in drug discovery: it requires physiological ATP concentrations to accurately model inhibitor potency, its low intrinsic activity demands sensitive detection, and covalent or allosteric inhibitor characterization is obscured by endpoint-only formats such as ADP-Glo or HTRF. Radiometric assays offer sensitivity but generate radioactive waste and lack temporal resolution. PhosphoSens continuous kinetic technology addresses these issues by generating real-time fluorescent progress curves at physiological ATP, enabling direct measurement of kinact/KI for covalent inhibitors, accurate Km-ATP determination, and detection of slow-binding kinetics. The no-antibody format eliminates cross-reactivity artifacts common in HTRF, while the continuous readout reveals inhibitor mechanism of action and compound-induced enzyme activation or cooperativity that endpoint assays fundamentally miss.
Key Pathways:
LKB1-AMPK signaling
Neuronal polarity and axonogenesis
DNA damage checkpoint
Tau phosphorylation and neurodegeneration
Talk to a Scientist
Have questions about BRSK1 assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time BRSK1 Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative BRSK1 activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0900
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0900)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0900
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for BRSK1?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteTRF BRSK1 Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative BRSK1 activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0900
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0900)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0900
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for BRSK1?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for BRSK1
Wild-type and mutant forms of BRSK1 for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
BRSK1, Active Full Length
Mouse • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-B13-10G
- Mutation: Full Length
- Species: Mouse
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~118 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: BRSK1 Assays
What substrate does AssayQuant use to measure BRSK1 activity with PhosphoSens?
AssayQuant offers optimized CSox-based peptide substrates derived from validated BRSK1 phosphorylation sequences, including tau-derived motifs. These substrates incorporate a chelation-enhanced fluorophore that produces a real-time fluorescent signal upon phosphorylation, enabling continuous monitoring without secondary reagents or antibodies.
Can the PhosphoSens BRSK1 assay be used to characterize covalent inhibitors?
Yes. Because PhosphoSens generates full progress curves in real time, it directly captures the kinetics of covalent inactivation, allowing determination of kinact and KI from a single experiment. This capability is not accessible with endpoint methods such as ADP-Glo or HTRF, which only report activity at a fixed time point and can produce misleading IC50 values for covalent or slow-binding compounds.
What ATP concentration is recommended for BRSK1 assays, and why does it matter?
AssayQuant recommends running BRSK1 assays at or near the physiological ATP concentration (1-2 mM) to accurately reflect inhibitor potency in a cellular context. Competitive ATP inhibitors tested at artificially low ATP concentrations will appear more potent than they truly are, inflating SAR confidence; PhosphoSens sensitivity allows robust signal generation even under these physiologically relevant conditions.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ BRSK1 Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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