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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About BRSK2
BRSK2 (Brain-specific serine/threonine kinase 2), also known as SAD-A, belongs to the CAMK-like (CAMKL) superfamily and is closely related to BRSK1, MARK, and AMPK family kinases. Activated by LKB1-mediated phosphorylation on its T-loop threonine (Thr174), BRSK2 is a master regulator of neuronal polarity, establishing axon-dendrite identity during neurodevelopment. Key substrates include tau, MAP2, and components of the WNT signaling pathway including Dvl proteins. Beyond the CNS, BRSK2 plays roles in pancreatic beta-cell function, insulin secretion, and cell cycle regulation via CDC25 phosphorylation. Dysregulation of BRSK2 has been linked to neurodevelopmental disorders, autism spectrum disorder, schizophrenia, and metabolic diseases. Somatic mutations and altered expression are observed in colorectal, breast, and pancreatic cancers, implicating BRSK2 as an oncology target. Its position downstream of the tumor suppressor LKB1 further elevates its therapeutic relevance in precision oncology and neuroscience drug discovery programs.
Why PhosphoSens for BRSK2?
BRSK2 presents several assay challenges: low intrinsic activity in recombinant preparations, requirement for LKB1-dependent activation, and sensitivity to ATP concentration that complicates conventional endpoint assays. ADP-Glo and HTRF methods require fixed endpoint reads, missing the early progress curve kinetics critical for detecting partial inhibitors or covalent warheads targeting BRSK2. Radiometric assays add throughput and safety burdens. AssayQuant's PhosphoSens CSox-based peptide substrates enable continuous, real-time fluorescence monitoring of BRSK2 activity at physiological ATP concentrations, generating complete progress curves from a single reaction. This approach captures accurate kinact/KI for covalent inhibitor characterization, eliminates endpoint sampling artifacts, and enables robust Km and kcat determination — making BRSK2 SAR campaigns faster, more reproducible, and biologically relevant compared to legacy detection technologies.
Key Pathways:
LKB1-AMPK signaling
Neuronal polarity establishment
WNT/beta-catenin signaling
Cell cycle regulation
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Have questions about BRSK2 assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time BRSK2 Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative BRSK2 activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0900
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0900)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0900
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
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Request a QuoteTRF BRSK2 Screening Assays
Robust endpoint TRF assays optimized for high-throughput screening and quantitative BRSK2 activity measurement in plate-based workflows.
Assay Format: Time-resolved fluorescence (TRF, endpoint)
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.
Learn more about PhosphoSens-Red →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Red Kinase Activity Kit - AQT0900
Ready-to-use TRF assay kits containing substrate and essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0900)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
- Europium Detection Solution
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $455
Substrate
PhosphoSens Kinase Substrate AQT0900
Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for BRSK2?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for BRSK2
Wild-type and mutant forms of BRSK2 for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
BRSK2, Active Full Length
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-B14-10G
- Mutation: Full Length
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~108 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$348.00
Request a QuoteFrequently Asked Questions: BRSK2 Assays
What substrate does AssayQuant offer for BRSK2 kinase assays?
AssayQuant provides PhosphoSens CSox-based peptide substrates optimized for BRSK2 activity detection in continuous fluorescence format. These substrates report phosphorylation in real time without antibodies or coupled enzyme systems, enabling accurate kinetic parameter determination at physiological ATP concentrations.
How does PhosphoSens handle the low activity typically observed with recombinant BRSK2?
PhosphoSens continuous assays generate full progress curves that can detect low-turnover kinase activity with high sensitivity, avoiding the signal compression inherent in endpoint assays. The real-time format allows reaction monitoring to be extended and initial velocity windows to be precisely identified, making it especially suited to low-activity BRSK2 preparations.
Can the PhosphoSens BRSK2 assay be used for covalent inhibitor characterization?
Yes. The continuous progress curve format is ideal for determining kinact and KI for covalent BRSK2 inhibitors, parameters that cannot be reliably extracted from single-timepoint endpoint assays. PhosphoSens monitors the time-dependent loss of enzyme activity in real time, providing mechanistic resolution that ADP-Glo and HTRF methods cannot deliver.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ BRSK2 Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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