Scientists today must deliver breakthroughs in technically challenging fields with flat budgets. Our PhosphoSens assay technology is designed to help you do more with less.
Developed by the renowned Imperiali Laboratory at MIT, our patented technologies are exclusively licensed to AssayQuant. Our team of experts leverages their vast assay development knowledge to provide robust products that enable breakthrough discoveries and boost productivity, ultimately benefiting human health.
Our product portfolio using the Sulfonamido-oxine (Sox) fluorophore for detection of protein kinase and phosphatase activity is commercialized under the brand name PhosphoSens. Sox was designed to be small and minimally hydrophobic and is well-tolerated across diverse sequences and targets.
By harnessing chelation-enhanced fluorescence (ChEF) with improved sulfonamido-oxine (Sox) chromophore technology for peptide or protein substrates, we have created a simple yet powerful method to measure the activity of protein kinases or phosphatases using a direct and homogeneous format that provides more information with less effort and accommodates any ATP concentration or sample type. The continuous (kinetic) format is ideal for elucidating drug mechanism of action, potency and profiling, while the Endpoint/Red format is ideal for HTS and SAR. One can switch between formats with the same sensor, to provide seamless coverage across the entire discovery and drug development process.
Most of the 30-plus commercially available kinase assay platforms use antibodies, coupled reactions, indirect measurements, radioactivity or complex instrumentation for detection. These and other limitations have slowed progress and prevented scientific discovery. Our recent advancements in continuous protein kinase or phosphatase activity assays accelerate discovery by providing better information with reduced effort.
AssayQuant has created a robust, high-throughput process for generating superior and fit-for-purpose Sox-based substrates starting with a physiologically relevant or known substrate. Our approach integrates the improved CSox technology from the Imperiali laboratory that allows flanking sequence on either side of the Sox chromophore. These optimized CSox substrates can be used in kinetic or endpoint assays. We have designed both highly generic and highly selective sensors.
The PhosphoSens platform delivers flexibility with assay components (use a wide range of ATP, substrate, activators or inhibitors), assay volume, plate configurations (96, 384 or 1536-well), microplate readers and sample types (purified enzyme or crude lysate) – all with a simple to use (add & read) and highly quantitative format that we offer in 3 variations:
The kinetic format captures the complete progress curve (fluorescence intensity over time) for every condition and with no additional handling. The endpoint format can use a stop solution to allow processing of plates in batch mode to provide higher-throughput.