AQT0663 PhosphoSens® Tyrosine Kinase Substrate
A PhosphoSens® tyrosine kinase substrate validated for 7 kinases (4 wild-type, 3 mutant), including BTK, JAK1 (JH1), JAK1 (JH1JH2). Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0663
- Enzyme class
- Kinase
- Residue specificity
- Tyrosine
- Validated kinases
- 7
- Assay formats
- Kinetic · 3 Red · 5
- Kinase families
- 3
Kinases validated with AQT0663
Every kinase below has a validated PhosphoSens® assay using AQT0663. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0663 | Link |
|---|---|---|---|---|
| BTK | Wild-type | Tec | Red | View assay → |
| EGFR [C797S/L858R] | Mutant | EGFR | Red | View assay → |
| EGFR [del746-750/C797S/T790M] | Mutant | EGFR | Red | View assay → |
| EGFR [T790M/L858R] | Mutant | EGFR | Red | View assay → |
| JAK1 (JH1) | Wild-type | Jak | Kinetic | View assay → |
| JAK1 (JH1JH2) | Wild-type | Jak | Kinetic | View assay → |
| TYK2 (JH1) | Wild-type | Jak | KineticRed | View assay → |
No kinases match that search.
Bulk substrate
AQT0663 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0663 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 7 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0663 validated for?
AQT0663 is validated for 7 kinases (4 wild-type and 3 mutant), including BTK, JAK1 (JH1), JAK1 (JH1JH2). See the full list above.
Which PhosphoSens assay formats use AQT0663?
AQT0663 is the PhosphoSens-Kinetic substrate for 3 kinases. It is the PhosphoSens-Red substrate for 5 kinases.
What sizes is AQT0663 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.