AQT0101 PhosphoSens® Tyrosine Kinase Substrate

A PhosphoSens® tyrosine kinase substrate validated for 64 kinases (2 wild-type, 62 mutant), including BTK, ITK. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.

Catalog number
AQT0101
Enzyme class
Kinase
Residue specificity
Tyrosine
Validated kinases
64
Assay formats
Kinetic · 64
Kinase families
9

Kinases validated with AQT0101

Every kinase below has a validated PhosphoSens® assay using AQT0101. Select a target to see its kits, recombinant enzyme, pricing and protocols.

KinaseTypeFamilyFormat with AQT0101Link
ALK [C1156Y] Mutant ALK Kinetic View assay →
ALK [F1174L] Mutant ALK Kinetic View assay →
ALK [G1269A] Mutant ALK Kinetic View assay →
ALK [G1269S] Mutant ALK Kinetic View assay →
ALK [L1196M] Mutant ALK Kinetic View assay →
ALK [R1275Q] Mutant ALK Kinetic View assay →
ALK [S1206R] Mutant ALK Kinetic View assay →
ALK [T1151-L1152insT] Mutant ALK Kinetic View assay →
ALK-NPM1 Mutant ALK Kinetic View assay →
AXL [R499C] Mutant Axl Kinetic View assay →
BTK Wild-type Tec Kinetic View assay →
BTK [C481R] Mutant TEK Kinetic View assay →
BTK [C481S] Mutant Tec Kinetic View assay →
BTK [P190K] Mutant TEK Kinetic View assay →
BTK [T474I] Mutant TEK Kinetic View assay →
BTK [Y485F] Mutant TEK Kinetic View assay →
FGFR1 [V561M] Mutant FGFR Kinetic View assay →
FGFR2 [C491A] Mutant FGFR Kinetic View assay →
FGFR2 [C491F] Mutant FGFR Kinetic View assay →
FGFR2 [E565A] Mutant FGFR Kinetic View assay →
FGFR2 [K641R] Mutant FGFR Kinetic View assay →
FGFR2 [N549K] Mutant FGFR Kinetic View assay →
FGFR2 [V564I] Mutant FGFR Kinetic View assay →
FGFR2 [V564L] Mutant FGFR Kinetic View assay →
FGFR3 [G697C] Mutant FGFR Kinetic View assay →
FGFR3 [K650E] Mutant FGFR Kinetic View assay →
FGFR3 [K650M] Mutant FGFR Kinetic View assay →
FGFR3 [V555L] Mutant FGFR Kinetic View assay →
FGFR4 [V550E] Mutant FGFR Kinetic View assay →
FGFR4 [V550L] Mutant FGFR Kinetic View assay →
FGFR4 [V550M] Mutant FGFR Kinetic View assay →
FLT3 [D835Y] Mutant PDGFR Kinetic View assay →
FLT3 [F691L D835Y] Mutant PDGFR Kinetic View assay →
FLT3 [ITD] Mutant PDGFR Kinetic View assay →
FLT3 [N676K] Mutant PDGFR Kinetic View assay →
ITK Wild-type Tec Kinetic View assay →
KIF5B-RET [V804L] Mutant Ret Kinetic View assay →
KIT [d557-558/Y823D] Mutant PDGFR Kinetic View assay →
KIT [d557-558] Mutant PDGFR Kinetic View assay →
KIT [D816V] Mutant PDGFR Kinetic View assay →
KIT [D820E] Mutant PDGFR Kinetic View assay →
KIT [V559D/T670I] Mutant PDGFR Kinetic View assay →
KIT [V559D/V654A] Mutant PDGFR Kinetic View assay →
KIT [V560G/D816V] Mutant PDGFR Kinetic View assay →
KIT [V654A] Mutant PDGFR Kinetic View assay →
NTRK1 [F589L] Mutant Trk Kinetic View assay →
NTRK1 [G595R G667A] Mutant Trk Kinetic View assay →
NTRK1 [G595R G667C] Mutant Trk Kinetic View assay →
NTRK1 [G595R G667S] Mutant Trk Kinetic View assay →
NTRK1 [G667A] Mutant Trk Kinetic View assay →
NTRK1 [G667C] Mutant Trk Kinetic View assay →
NTRK1 [G667S] Mutant Trk Kinetic View assay →
NTRK1 [L657M] Mutant Trk Kinetic View assay →
RET [G810C/M918T] Mutant Ret Kinetic View assay →
RET [L730I] Mutant Ret Kinetic View assay →
RET [S904A] Mutant Ret Kinetic View assay →
RET [V738A] Mutant Ret Kinetic View assay →
RET [V804M] Mutant Ret Kinetic View assay →
RET [Y806H] Mutant Ret Kinetic View assay →
RET [Y806N] Mutant Ret Kinetic View assay →
ROS1 [G2032R] Mutant Sev Kinetic View assay →
ROS1 [G2101A] Mutant Sev Kinetic View assay →
ROS1 [G2101C] Mutant Sev Kinetic View assay →
ROS1 [L2086F] Mutant Sev Kinetic View assay →

Bulk substrate

AQT0101 PhosphoSens® Kinase Substrate

Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0101 are on each target's assay page.

Price: $2795 USD

How PhosphoSens® substrates work

PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.

  • Real-time progress curves, not single end-points
  • Run at physiologically relevant ATP concentrations
  • No antibodies, coupling enzymes, wash steps or radioactivity
  • One substrate across 64 kinases simplifies selectivity and panel work
Learn about PhosphoSens® technology →

Frequently asked questions

Which kinases is AQT0101 validated for?

AQT0101 is validated for 64 kinases (2 wild-type and 62 mutant), including BTK, ITK. See the full list above.

Which PhosphoSens assay formats use AQT0101?

AQT0101 is the PhosphoSens-Kinetic substrate for 64 kinases.

What sizes is AQT0101 available in?

1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.

Request a Quote