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Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.
About LRRK2
LRRK2 (Leucine-Rich Repeat Kinase 2) belongs to the LRRK family of large, multidomain Roco GTPases that uniquely combine both GTPase and serine/threonine kinase activities within a single polypeptide. LRRK2 is activated through autophosphorylation and Rab8A/Rab10-mediated feedback loops, and its activity is tightly regulated by 14-3-3 protein binding and PP1 phosphatase. Key substrates include Rab GTPases (Rab8A, Rab10, Rab35) at conserved threonine residues, as well as NSF and RILPL1/2, linking LRRK2 to endolysosomal trafficking, autophagy, and ciliogenesis. LRRK2 is centrally implicated in Parkinson's disease (PD); gain-of-function mutations such as G2019S (the most prevalent familial PD mutation) hyperactivate kinase activity by 2–3 fold. LRRK2 inhibition has emerged as a compelling therapeutic strategy for both familial and sporadic PD, and LRRK2 variants also associate with Crohn's disease and leprosy susceptibility, broadening its disease relevance beyond neurodegeneration.
Why PhosphoSens for LRRK2?
LRRK2 presents significant assay challenges: it is a large (286 kDa), low-abundance kinase requiring careful handling to preserve activity, and its preferred substrates are Rab GTPases with moderate catalytic efficiency. Traditional endpoint assays such as ADP-Glo and HTRF require quenching steps that can miss transient activity changes, introduce variability, and fail to capture the full kinetic profile needed for covalent inhibitor characterization. Radiometric assays add safety and throughput burdens. PhosphoSens continuous fluorescent kinase assays overcome these limitations by generating real-time progress curves at physiological ATP concentrations, enabling accurate Km(ATP) determination, detection of covalent kinact/KI parameters for irreversible inhibitors, and early identification of assay artifacts such as compound fluorescence interference or substrate depletion — all critical for prosecuting LRRK2 drug discovery campaigns with high fidelity.
Key Pathways:
Rab GTPase trafficking
mTORC1 signaling
autophagy-lysosome pathway
neuroinflammation signaling
Talk to a Scientist
Have questions about LRRK2 assay design, selectivity panels, or covalent inhibitor characterization?
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How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time LRRK2 Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative LRRK2 activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0615
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0615)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0615
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for LRRK2?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteNo PhosphoSens-Red format is currently available for LRRK2.
Ready to request pricing for LRRK2?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
Request a QuoteActive Recombinant Enzymes for LRRK2
Wild-type and mutant forms of LRRK2 for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.
LRRK2, Active
Human • Baculovirus-Insect Cells
Technical Specifications
- Catalog: AQT-L10-11G
- Species: Human
- Expression System: Baculovirus-Insect Cells
- Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
- Molecular Weight: ~210 kDa
- Purity/Activity: For specific information on a given lot, see related technical data sheet.
- Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:
Price: $$488.00
Request a QuoteFrequently Asked Questions: LRRK2 Assays
What substrate does AssayQuant recommend for LRRK2 kinase assays?
AssayQuant offers PhosphoSens CSox-based peptide substrates optimized for LRRK2, derived from validated Rab GTPase phosphorylation sites. These substrates enable continuous, real-time monitoring of LRRK2 activity without antibodies or secondary detection reagents. Substrate selection guidance is available from our scientific team to match your specific assay format and throughput requirements.
Can PhosphoSens assays measure covalent LRRK2 inhibitor kinetics?
Yes. Because PhosphoSens generates continuous fluorescent progress curves, it is uniquely suited to capture the time-dependent inhibition profiles required to determine kinact and KI for covalent or irreversible LRRK2 inhibitors. This cannot be reliably accomplished with single-timepoint endpoint assays such as ADP-Glo or HTRF, which may underestimate or misrepresent covalent inhibitor potency.
What ATP concentration is used in AssayQuant LRRK2 assays, and why does it matter?
PhosphoSens LRRK2 assays can be run at physiological ATP concentrations (typically 1 mM), closely mimicking cellular conditions and yielding IC50 values more predictive of in vivo potency. Many competing platforms use artificially low ATP to boost signal, which inflates apparent compound potency and reduces translational relevance u2014 a critical consideration for LRRK2 drug discovery programs targeting CNS indications.
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ LRRK2 Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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