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PhosphoSens GSK3B [C199A] Protein Kinase Assays, Substrates & Recombinant Enzymes
Also known as: GSK3B • GSK3beta • GSK-3B
![PhosphoSens GSK3B [C199A] kinase assay kit — continuous real-time fluorescence](https://www.assayquant.com/hs-fs/hubfs/PhosphoSens%20Packaging%20C%20(3).jpg?width=450&name=PhosphoSens%20Packaging%20C%20(3).jpg)
About GSK3B [C199A]
Glycogen synthase kinase 3 beta (GSK3B) is a serine/threonine kinase belonging to the CMGC superfamily and the GSK family, closely related to GSK3A. Unlike most kinases, GSK3B is constitutively active in resting cells and is inactivated upon stimulation. Activation is regulated through inhibitory phosphorylation at Ser9 by upstream kinases including AKT, PKA, and p90RSK, or through activating phosphorylation at Tyr216. GSK3B phosphorylates a remarkably broad substrate repertoire — including glycogen synthase, beta-catenin, tau, c-Myc, and CRMP2 — making it a central node in PI3K/AKT, Wnt/beta-catenin, Hedgehog, and insulin signaling pathways. Dysregulation of GSK3B is implicated in Alzheimer's disease (tau hyperphosphorylation), type 2 diabetes, bipolar disorder, cancer, and neurodegeneration. Its role in multiple oncogenic and neuropathological cascades, combined with its druggability, makes GSK3B one of the most intensively pursued therapeutic targets in kinase drug discovery.
Why PhosphoSens for GSK3B [C199A]?
GSK3B presents several assay challenges: its constitutive activity requires precise control of inhibitory pre-phosphorylation states, and many GSK3B substrates require prior priming phosphorylation, complicating endpoint-based formats. ADP-Glo and HTRF assays capture only a single time point, missing kinetic nuances of slow-binding or covalent inhibitors and introducing artifacts from substrate depletion or inner-filter effects. Radiometric assays add radioactive waste concerns and poor throughput. PhosphoSens continuous kinase assays overcome these limitations by providing real-time fluorescent progress curves at physiological ATP concentrations, enabling accurate Km(ATP) determination critical for selectivity profiling. Full kinetic curves allow confident measurement of kinact/KI for covalent inhibitors and early detection of non-linear kinetics indicative of tight-binding or allosteric inhibition — delivering richer SAR data per experiment with significantly reduced compound consumption.
Key Pathways:
PI3K/AKT/mTOR signaling
Wnt/beta-catenin signaling
Insulin signaling
Tau phosphorylation and neurodegeneration
Talk to a Scientist
Have questions about GSK3B [C199A] assay design, selectivity panels, or covalent inhibitor characterization?
Ask Our Scientists →
How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.
Real-Time GSK3B [C199A] Activity Assays
Continuous, real-time fluorescent assays optimized for quantitative GSK3B [C199A] activity measurements, IC50 determination, and mechanistic studies.
Assay Format: Continuous kinetic fluorescence (real-time)
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.
Learn more about PhosphoSens-Kinetic →Need pricing or availability? Select a kit or substrate to request a quote below.
Kits
PhosphoSens-Kinetic Kinase Activity Kit - AQT0157
Ready-to-use assay kits containing substrate and all essential reagents.
Kit Contents
- Optimized PhosphoSens® substrate (AQT0157)
- ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes
Price: $425
Substrate
PhosphoSens Kinase Substrate AQT0157
Bulk PhosphoSens® substrate for assay development and high-throughput workflows.
Specifications
Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs
Price: $2795
Ready to request pricing for GSK3B [C199A]?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
No PhosphoSens-Red format is currently available for GSK3B [C199A].
Ready to request pricing for GSK3B [C199A]?
Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.
No recombinant enzymes are currently available for GSK3B [C199A].
Data & Resources
Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.
Sample Report: AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
- Enzyme titration and linearity
- Sensor peptide substrate titration and Km determination
- ATP titration and Km determination
- DMSO titration and assay tolerance
- Inhibitor titration and IC50 determination
Protocol
Inhibitor IC50 Protocol
See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.
Download Protocol →Webinar
Webinar: Continuous Assay Validation
Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.
Watch the Webinar →Service
KinSight™ GSK3B [C199A] Profiling
Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.
Learn about KinSight →Stay Informed
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