PhosphoSens GRK4 Protein Kinase Assays, Substrates & Recombinant Enzymes

Continuous, real-time assays that directly quantify GRK4 catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.

  • Real-time kinetic or TRF endpoint formats
  • Optimized substrates and assay-ready kits
  • Compatible with physiological ATP and cofactors

Designed for: drug discovery, lead optimization, and mechanistic kinase research teams

 

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PhosphoSens GRK4 kinase assay kit — continuous real-time fluorescence

How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.

Real-Time GRK4 Activity Assays

Continuous, real-time fluorescent assays optimized for quantitative GRK4 activity measurements, IC50 determination, and mechanistic studies.

Assay Format: Continuous kinetic fluorescence (real-time)

PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.

Learn more about PhosphoSens-Kinetic →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Kinetic Kinase Activity Kit - AQT0641

Ready-to-use assay kits containing substrate and all essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0641)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $425

Substrate

PhosphoSens Kinase Substrate AQT0641

Bulk PhosphoSens® substrate for assay development and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for GRK4?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

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TRF GRK4 Screening Assays

Robust endpoint TRF assays optimized for high-throughput screening and quantitative GRK4 activity measurement in plate-based workflows.

Assay Format: Time-resolved fluorescence (TRF, endpoint)

PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.

Learn more about PhosphoSens-Red →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Red Kinase Activity Kit - AQT0641

Ready-to-use TRF assay kits containing substrate and essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0641)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
  • Europium Detection Solution

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $455

Substrate

PhosphoSens Kinase Substrate AQT0641

Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for GRK4?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

Request a Quote

No recombinant enzymes are currently available for GRK4.

🔬 150+ peer-reviewed citations · 🏛️ MIT spinout technology · ⚗️ PhosphoSens® validated by independent labs worldwide · Browse technical articles →

Data & Resources

Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.

Sample Report: AQT0734 - EGFR Assay Validation Report

Sample Report: AQT0734 - EGFR Assay Validation Report

Each validation report provides experimental conditions and data showing:

  • Enzyme titration and linearity
  • Sensor peptide substrate titration and Km determination
  • ATP titration and Km determination
  • DMSO titration and assay tolerance
  • Inhibitor titration and IC50 determination
Download the Report

Protocol

Inhibitor IC50 Protocol

See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.

Download Protocol →

Webinar

Webinar: Continuous Assay Validation

Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.

Watch the Webinar →

Service

KinSight™ GRK4 Profiling

Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.

Learn about KinSight →

Still have questions about our products?

Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.

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