AQT0462 PhosphoSens® Serine/threonine Kinase Substrate
A PhosphoSens® serine/threonine kinase substrate validated for 12 kinases, including PKN1, PKN2, PRKCA. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0462
- Enzyme class
- Kinase
- Residue specificity
- Serine/threonine
- Validated kinases
- 12
- Assay formats
- Kinetic · 6 Red · 11
- Kinase families
- 2
Kinases validated with AQT0462
Every kinase below has a validated PhosphoSens® assay using AQT0462. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0462 | Link |
|---|---|---|---|---|
| PKN1 | Wild-type | PKN | Kinetic | View assay → |
| PKN2 | Wild-type | PKN | Red | View assay → |
| PRKCA | Wild-type | PKC | Red | View assay → |
| PRKCB1 | Wild-type | PKC | Red | View assay → |
| PRKCB2 | Wild-type | PKC | KineticRed | View assay → |
| PRKCD | Wild-type | PKC | KineticRed | View assay → |
| PRKCE | Wild-type | PKC | Red | View assay → |
| PRKCG | Wild-type | PKC | Red | View assay → |
| PRKCH | Wild-type | PKC | KineticRed | View assay → |
| PRKCI | Wild-type | PKC | KineticRed | View assay → |
| PRKCQ | Wild-type | PKC | Red | View assay → |
| PRKCZ | Wild-type | PKC | KineticRed | View assay → |
No kinases match that search.
Bulk substrate
AQT0462 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0462 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 12 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0462 validated for?
AQT0462 is validated for 12 kinases, including PKN1, PKN2, PRKCA. See the full list above.
Which PhosphoSens assay formats use AQT0462?
AQT0462 is the PhosphoSens-Kinetic substrate for 6 kinases. It is the PhosphoSens-Red substrate for 11 kinases.
What sizes is AQT0462 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.