AQT0349 PhosphoSens® Serine/threonine Kinase Substrate
A PhosphoSens® serine/threonine kinase substrate validated for 5 kinases, including BUB1, EEF2K, EIF2AK4. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0349
- Enzyme class
- Kinase
- Residue specificity
- Serine/threonine
- Validated kinases
- 5
- Assay formats
- Kinetic · 4 Red · 3
- Kinase families
- 4
Kinases validated with AQT0349
Every kinase below has a validated PhosphoSens® assay using AQT0349. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0349 | Link |
|---|---|---|---|---|
| BUB1 | Wild-type | BUB | Red | View assay → |
| EEF2K | Wild-type | Alpha | Kinetic | View assay → |
| EIF2AK4 | Wild-type | PEK | Kinetic | View assay → |
| NEK8 | Wild-type | NEK | KineticRed | View assay → |
| TRPM7 | Wild-type | Alpha | KineticRed | View assay → |
No kinases match that search.
Bulk substrate
AQT0349 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0349 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 5 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0349 validated for?
AQT0349 is validated for 5 kinases, including BUB1, EEF2K, EIF2AK4. See the full list above.
Which PhosphoSens assay formats use AQT0349?
AQT0349 is the PhosphoSens-Kinetic substrate for 4 kinases. It is the PhosphoSens-Red substrate for 3 kinases.
What sizes is AQT0349 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.