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Lysate Kinase Activity Assays

PhosphoSens Lysate Assays directly quantify functional kinase activity in cell and tissue lysates. Unlike phosphoprotein methods that only measure phosphorylation state as a proxy, PhosphoSens captures true enzymatic activity at the substrate level, delivering real-time progress curves in a biologically relevant context.

Every well produces a complete activity profile — not just a snapshot — so you can clearly see kinase function, inhibitor dynamics, and pathway modulation in the context of native biology.

Learn About PhosphoSens-Lysate Assay Technology >
Shop By Target

Shop Cell Lysate Kinase Assays By Target

Use the table below to find the Cell Lysate Kinase Assay that matches your target of interest. Each Kinase-Selective Lysate Substrate is identified by its unique AQT code (e.g., AQT1076).
To place an order, simply email us with the desired product format:

  • CSLS-AQTxxxx-KL-100: 100-assay Lysate Kit
  • CSLS-AQTxxxx-KL-1000: 1,000-assay Lysate Kit
  • CSLS-AQTxxxx-B-01: 1 mg of selective lysate-compatible substrate
Custom configurations and bulk reagents available upon request. 
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Target Name HGNC Approved Full Name Kinase-Selective Lysate Substrate Sensor Peptide MW (g/mol) Assay Validation Report Target Specific Protocol
ERK1/2 mitogen-activated protein kinase (MAPK3/1) AQT1076 2372.95 LINK > LINK >
AKT1/2/3 AKT Serine/Threonine Kinase 1/2/3 AQT0982 3204.69 LINK > LINK >
GSK3A/B glycogen synthase kinase 3 alpha/beta AQT1121 2005.92 LINK > LINK >
DNA-PK protein kinase, DNA-activated, catalytic subunit AQT0440 1963.1 LINK > LINK >
JNK1/2/3 mitogen-activated protein kinase8/9/10 AQT1196 3162.30 LINK > LINK >
p38A/B/D/G mitogen-activated protein kinase11/12/13/14 AQT1280 2167.44 LINK > LINK >
CDK1/2/3/5 Cyclin-dependent kinase 1/2/3/5 AQT1271 948.84 LINK > Please Inquire
CDK2/CycE1 Cyclin-dependent kinase 2 & Cyclin E1 AQT1170 1209.42 LINK > Please Inquire

Download Our Comprehensive Cell Lysate Kinase Assay Kit Components Tables

Looking for more detail? Download our complete product list, which includes component tables for each lysate assay.

Resources

PhosphoSens Lysate Technology

Direct, Real-Time Detection of Kinase Activity in Complex Samples

20250709 AssayQuant Workflow Graphic 03-1

PhosphoSens Lysate Assays directly quantify functional kinase activity in cell and tissue lysates. Unlike phosphoprotein methods that only measure phosphorylation state as a proxy, PhosphoSens captures true enzymatic activity at the substrate level, delivering real-time progress curves in a biologically relevant context.

View PhosphoSens-Lysate Technology Page →
Application Data

Kinase Activity Across Real Cell Models

See how kinase activity changes across multiple cell lines, conditions, and stimuli — and why functional assays reveal dynamics that phospho readouts miss.

Explore Application Data
Educational Content

Phosphorylation ≠ Activity

Most kinase assays infer activity from phospho-status. Learn why catalytic function and phosphorylation often diverge — and how PhosphoSens measures real activity.

Stop inferring activity & start measuring it
Products & Pricing

Quantify Endogenous Kinase Activity and Signaling in Cell Lysates and Tissue Homogenates - No Antibodies, No Washes, Just a Continuous Signal

PhosphoSens Packaging C (3)

Flexible Configurations Built Around Our Substrates For Your Applications

Cell Lysate Assay Kit (100 Assays)

AQTxxxx-KL-100

$495

Designed for streamlined technology evaluation, each kit includes sufficient volumes of substrate, reaction reagents, cell extraction reagents and controls to perform 100 reactions at 15 μM substrate in a 384-well plate (25 μL reaction volume).

Includes:

  • PhosphoSens Kinase-Selective Lysate Substrate
  • ATP, EGTA, DTT, Enzyme Reaction Buffer and Enzyme Dilution Buffer
  • PhosphoPreserve Cell Extraction Buffer, Protease Inhibitor and Phosphatase Inhibitor Cocktails
  • PhosphoControl Peptide, Recombinant Enzyme, Cell Lysate and Reference Compound 

Cell Lysate Assay Kit (1,000 Assays)

AQTxxxx-KL-1000

$3,195

Designed for streamlined technology evaluation, each kit includes sufficient volumes of substrate, reaction reagents and cell extraction reagents to perform 1,000 reactions at 15 μM substrate in a 384-well plate (25 μL reaction volume).

Includes:

  • PhosphoSens Kinase-Selective Lysate Substrate
  • ATP, EGTA, DTT, Enzyme Reaction Buffer and Enzyme Dilution Buffer
  • PhosphoPreserve Cell Extraction Buffer, Protease Inhibitor and Phosphatase Inhibitor Cocktails

Kinase-Selective Lysate Substrate (1mg)

AQTxxxx-L-01

$3,500

AssayQuant's Cell Lysate Kinase Assays combine PhosphoSens Detection Technology with high-throughput peptide synthesis to design sensor peptide substrates that are highly selective for your targets of interest.

Each Kinase-Selective Lysate Substrate can be purchased as lyophilized 1mg aliquots. Each mg is sufficient for 1,500 reactions at 15 μM substrate in a 384-well plate (25 μL reaction volume).

Bulk Pricing available.

 

Cell Lysate Assay Reagent Pack

AQT-LRP

$600

Designed for end user flexibility, incorporate our optimized reagents into your workflow. These reagent packs include sufficient volumes of reaction reagents and cell extraction reagents to perform 2,000 reactions in a 384-well plate (25 μL reaction volume).

Includes:

  • ATP, EGTA, DTT, Enzyme Reaction Buffer and Enzyme Dilution Buffer
  • PhosphoPreserve Cell Extraction Buffer, Protease Inhibitor and Phosphatase Inhibitor Cocktails

Cell Lysate Assay Controls

Contact Us For Pricing

Ready to scale up from 100 assays but still want the positive controls we used to develop and validate the assay? 

Contact us at hello@assayquant.com and we will work with you to get the right amount of controls for your experiment. 

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