PhosphoSens EGFR Protein Kinase Assays, Substrates & Recombinant Enzymes

Continuous, real-time assays that directly quantify EGFR catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.

  • Real-time kinetic or TRF endpoint formats
  • Optimized substrates and assay-ready kits
  • Compatible with physiological ATP and cofactors

Designed for: drug discovery, lead optimization, and mechanistic kinase research teams

 

How pricing works:
How pricing works: Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.

Real-Time EGFR Activity Assays

Continuous, real-time fluorescent assays optimized for quantitative EGFR activity measurements, IC50 determination, and mechanistic studies.

Assay Format: Continuous kinetic fluorescence (real-time)

PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.

Learn more about PhosphoSens-Kinetic →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Kinetic Kinase Activity Kit - AQT0734

Ready-to-use assay kits containing substrate and all essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0734)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $425

Substrate

PhosphoSens Kinase Substrate AQT0734

Bulk PhosphoSens® substrate for assay development and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for EGFR?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

Request a Quote

TRF EGFR Screening Assays

Robust endpoint TRF assays optimized for high-throughput screening and quantitative EGFR activity measurement in plate-based workflows.

Assay Format: Time-resolved fluorescence (TRF, endpoint)

PhosphoSens-Red assays use europium-based time-resolved fluorescence detection to enable robust, plate-based quantification of enzyme activity, making them well suited for high-throughput screening workflows.

Learn more about PhosphoSens-Red →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Red Kinase Activity Kit - AQT0653

Ready-to-use TRF assay kits containing substrate and essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0653)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer
  • Europium Detection Solution

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $455

Substrate

PhosphoSens Kinase Substrate AQT0653

Bulk substrate for PhosphoSens-Red TRF assays and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for EGFR?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

Request a Quote

Active Recombinant Enzymes for EGFR

Wild-type and mutant forms of EGFR for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.

EGFR, Active

Human • Baculovirus-Insect Cells

Technical Specifications
  • Catalog: AQT-E10-112G
  • Species: Human
  • Expression System: Baculovirus-Insect Cells
  • Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
  • Molecular Weight: ~89-100kDa
  • Purity/Activity: For specific information on a given lot, see related technical data sheet.
  • Storage Conditions: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:

Price: $ $417.00

Request Quote

How many assays can I run per 1 mg of Sensor Peptide?

PhosphoSens Sensor Peptides range in molecular weight (MW) based on the size and sequence of the substrate. The average MW or a sensor peptide is 2,150 g/mol. This size Sensor Peptide will be re-suspended in approximately 450 µL of diluent. If running an assay in a 384-well plate, with a final well volume of 20 µL and Sensor Peptide concentration of 10 µM, you can run 2,250 assays!

How do I resuspend my lyophilized sensor peptide?

Reference the "Solubilization" section of the printed Certificate of Analysis (CofA) that is printed and shipped with every order. If you do not see this, please contact us at support@assayquant.com and we will share an electronic copy of your CofA. 

What is a reagent pack?

Reagent packs include optimized buffers, cofactors, and detection reagents required to run PhosphoSens® assays. They are used with bulk substrates or custom assay workflows.

About EGFR

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Key Pathways

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Biological Function

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Disease Relevance

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Mutants

D770_N771insNPG, C797S/L858R, del746-750/C797S/T790M, T790M/L858R, del746-750/C797S, del746-750/T790M, del746-750, L858R, L861Q, T790M/C797S/L858R, T790M/C797S/L858R, T790M

Data & Resources

Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.

Protocols & Manuals

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SDS & Certificates

Additional Resources

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Still have questions about our products?

Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.

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