Real-time kinase and phosphatase assays and services for drug discovery
PhosphoSens® sensor peptide substrates capture the full reaction curve in every well — revealing inhibitor mechanism, reversibility, and kinetics that a single endpoint read can't see.
Same EGFR reaction, two detection methods — one gives a full kinetic profile, the other gives a handful of points.
Find your target
Search accross 500+ kinase and 45+ phosphatase assays to explore AssayQuant’s product availability and services.
Publications
Enzyme targets
Of the top pharma companies use PhosphoSens
Tell us what stage you're at
Whether you're running assays in-house, outsourcing profiling, or still evaluating the approach — here's the fastest path.
Buy a kit
Browse the catalog and order kinase, phosphatase, or lysate assay kits directly. Choose continuous (FI) or time-resolved (TRF) format, validated for over 500 kinase and 35 phosphatase targets — ships in days.
Outsource it to our lab
Send us your compounds or targets. We run kinome and phosphatase selectivity profiling, potency (IC50/EC50), mechanism-of-action, time-dependent inhibition, and custom assay development.
Still researching
New to continuous kinetic assays? Get the capabilities brochure and the TDI workflow poster, walked through with real EGFR inhibitor data, before you commit to a kit or a KinSight engagement.
Supporting Every Phase of Preclinical Drug Discovery
From early screening through preclinical testing, AssayQuant accelerates drug discovery by turning kinetic insight into confident, data-driven decisions.
PhosphoSens Assays deliver direct, continuous measurement of kinase and phosphatase activity — converting phosphorylation events into real-time fluorescent signals for quantitative mechanistic insight. KinSight Services apply these same assays in our lab to measure compound potency, selectivity, and mechanism of inhibition across hundreds of enzyme targets. Together, these platforms enable faster, data-driven decisions from target validation through lead optimization.
Two Fit-for-Purpose Formats
Choose continuous (FI) for real-time kinetic insight or time-resolved (TRF) for screening applications.
Broad Sample Compatibility
Measure activity in recombinant enzyme systems or complex cell/tissue lysates.
Target
Validation
Confirm kinase and phosphatase targets driving key pathways.
High Throughput Screening
Screen compounds librraies using TRF readouts.
Lead
Identification
Characterize true inhibition and potency from kinetic data.
Lead
Optimization
Differentiate reversible vs. covalent inhibition and refine selectivity.
Preclinical
Testing
Evaluate activity and selectivity in cell or tissue lysates.
A Breakthrough Platform for Continuous Enzyme Activity Measurement
PhosphoSens is AssayQuant’s core technology platform that uses fluorescent sensor peptide substrates to track enzyme activity in real-time. Unlike traditional endpoint assays that only show a single measurement after stopping the reaction, PhosphoSens generates a progress curve in every well — giving you insight into reaction dynamics, inhibitor mechanism, and enzyme behaviour.
Gain a Competitive Edge with Real-Time Phosphorylation Dynamics
Characterizing targets and assessing compound potency, selectivity, and mechanism of action are critical steps in drug discovery, especially with the expanding focus on kinase inhibitors. Continuous assays simplify workflows, reduce variability, and deliver the data you need to make confident decisions — without separate experiments for reversibility or time-dependent inactivation.
Enzyme activity assay kits, substrates, and recombinant enzymes for drug discovery — built on PhosphoSens technology
AssayQuant's patented PhosphoSens technology powers a portfolio of over 700 unique enzyme-and-inhibitor assays for direct, real-time measurement of kinase and phosphatase activity. Our substrates and recombinant enzymes are available in flexible configurations — whether you're evaluating the technology in a single-plate format or running full high-throughput screens. With validated assays for more than 500 protein kinases, 35 phosphatases, and a catalog of 1,100+ highly active enzymes, we supply everything you need to advance inhibitor discovery.
Kinase Assays
Comprehensive kinase assay portfolio offering real-time activity and inhibition measurement with multiple product configurations.
Phosphatase Assays
Discover continuous, real-time phosphatase activity measurement with a wide selection of assays and customizable reagent options.
Lysate Assays
A direct, continuous fluorescence system for studying kinase activity and cell signaling in unfractionated lysates and tissue homogenates.
Recombinant Enzymes
Comprehensive selection of kinases and phosphatases designed for a wide range of applications, from basic research to preclinical drug discovery.
Kinase and phosphatase profiling services for potency, selectivity, and mechanism-of-action studies
AssayQuant's KinSight services deliver real-time kinetic insight into compound potency, selectivity, and mechanism of inhibition (MOI) using continuous enzyme activity assays built on our PhosphoSens technology platform. Whether you're evaluating inhibitor selectivity, confirming target engagement, or developing custom assays, KinSight provides quantitative data that reveal true enzyme kinetics — helping you make faster, more confident decisions in drug discovery.
Featured Services
Biochemical Kinase Screening and Profiling Services
Characterize compound selectivity and mechanism of action (MOA) against our panel of 400+ WT protein kinases.
Time-Dependent Inhibition Characterization
Quantify the kinetics behind inhibition with real-time data on inhibitor potency, reversibility, residence time and inactivation kinetics with confidence.
Frequently asked questions
What's the difference between continuous and endpoint kinase assays?
Endpoint assays stop the reaction and measure a single timepoint, giving you one data point per well. Continuous assays like PhosphoSens generate a full reaction progress curve in real time, revealing reaction dynamics, inhibitor mechanism, reversibility, and time-dependent inactivation kinetics that an endpoint read can't capture.
How many kinase and phosphatase targets does AssayQuant support?
AssayQuant offers validated assays for more than 500 protein kinases and 35 phosphatases, with a broader catalog of 1,100+ highly active recombinant enzymes available for custom and research use.
Should I buy a kit or use KinSight services?
If you're running assays in-house and want full control over the workflow, our kinase, phosphatase, and lysate kits ship directly. If you'd rather send us compounds or targets, KinSight services run profiling, potency, selectivity, MOI, and TDI studies in our lab and deliver the data back to you.
IC50s aren't enough to describe a time-dependent inhibitor
A proven three-step workflow for identifying and characterizing time-dependent kinase inhibitors using a continuous assay format — walked through with three real, approved EGFR drugs: Gefitinib (Iressa), Lapatinib (Tykerb), and Osimertinib (Tagrisso).
.jpg?width=2000&height=1953&name=AssayQuant%20A%20Proven%20Activity-based%20Workflow%20for%20the%20Identification%20and%20Characterization%20of%20Time-Dependent%20Kinase%20Inhibitors%20using%20a%20Continuous%20Assay%20Format%20(1).jpg "AssayQuant A Proven Activity-based Workflow for the Identification and Characterization of Time-Dependent Kinase Inhibitors using a Continuous Assay Format (1)")
Includes: TDI identification protocol · reversibility assessment · kinact/KI determination · full data on all three compounds
Have a target in mind? Let's talk kinetics.
Tell us what you're working on — we'll point you to the right assay, kit, or KinSight service.
