PhosphoSens HIPK4 Protein Kinase Assays, Substrates & Recombinant Enzymes

Continuous, real-time assays that directly quantify HIPK4 catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.

  • Real-time kinetic or TRF endpoint formats
  • Optimized substrates and assay-ready kits
  • Compatible with physiological ATP and cofactors

Designed for: drug discovery, lead optimization, and mechanistic kinase research teams

 

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PhosphoSens HIPK4 kinase assay kit — continuous real-time fluorescence

How pricing works:
Select a kit or substrate below, then request a quote. Bundle discounts apply automatically.

Real-Time HIPK4 Activity Assays

Continuous, real-time fluorescent assays optimized for quantitative HIPK4 activity measurements, IC50 determination, and mechanistic studies.

Assay Format: Continuous kinetic fluorescence (real-time)

PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously monitoring substrate phosphorylation or dephosphorylation in real time, generating a full progress curve in every well.

Learn more about PhosphoSens-Kinetic →

Need pricing or availability? Select a kit or substrate to request a quote below.

Kits

PhosphoSens-Kinetic Kinase Activity Kit - AQT0850

Ready-to-use assay kits containing substrate and all essential reagents.

Kit Contents
  • Optimized PhosphoSens® substrate (AQT0850)
  • ATP, DTT, EGTA, Reaction Buffer & Dilution Buffer

Automatically save 10% when bundling 10ug recombinant enzyme with your 1,000 assay kit. View enzymes

Price: $425

Substrate

PhosphoSens Kinase Substrate AQT0850

Bulk PhosphoSens® substrate for assay development and high-throughput workflows.

Specifications

Automatically save 10% when bundling Reagent Packs with your substrate. View Reagent Packs

Price: $2795

Ready to request pricing for HIPK4?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

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No PhosphoSens-Red format is currently available for HIPK4.

Ready to request pricing for HIPK4?

Select a kit, substrate, or enzyme above. Our team will confirm pricing, availability, and any applicable bundle discounts.

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Active Recombinant Enzymes for HIPK4

Wild-type and mutant forms of HIPK4 for assay development, kinase profiling, and mechanistic studies. Enzymes are supplied active and optimized for PhosphoSens® substrates.

HIPK4, Active Full Length

Human • Baculovirus-Insect Cells

Technical Specifications
  • Catalog: AQT-H06-10G
  • Mutation: Full Length
  • Species: Human
  • Expression System: Baculovirus-Insect Cells
  • Storage Buffer: Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50-300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25-1.0mM DTT, 0-0.1mM PMSF, 10-25% glycerol
  • Molecular Weight: ~110 kDa
  • Purity/Activity: For specific information on a given lot, see related technical data sheet.
  • Storage Conditions: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Available Sizes:

Price: $$348.00

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🔬 150+ peer-reviewed citations · 🏛️ MIT spinout technology · ⚗️ PhosphoSens® validated by independent labs worldwide · Browse technical articles →

Data & Resources

Explore data and documents to support your kinase and phosphatase experiments. Download sample data, protocols and other resources to see how our assays perform and to help you get started in your own lab. All validation data generated using PhosphoSens® assays under recommended conditions.

Sample Report: AQT0734 - EGFR Assay Validation Report

Sample Report: AQT0734 - EGFR Assay Validation Report

Each validation report provides experimental conditions and data showing:

  • Enzyme titration and linearity
  • Sensor peptide substrate titration and Km determination
  • ATP titration and Km determination
  • DMSO titration and assay tolerance
  • Inhibitor titration and IC50 determination
Download the Report

Protocol

Inhibitor IC50 Protocol

See how the PhosphoSens-Kinetic Assay can be used to find the IC50 of a kinase inhibitor.

Download Protocol →

Webinar

Webinar: Continuous Assay Validation

Discover how continuous assay formats power deep understanding of kinase function. See how PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and mechanistic characterization.

Watch the Webinar →

Service

KinSight™ HIPK4 Profiling

Need broader selectivity data? KinSight profiling runs your compounds across our full kinase panel under identical PhosphoSens conditions.

Learn about KinSight →

Still have questions about our products?

Our scientists can help you choose the right assay format, substrate, and enzyme for your experiment.

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