PhosphoSens EGFR Protein Kinase Assays, Substrates & Recombinant Enzymes
Continuous, real-time assays that directly quantify EGFR catalytic activity — generating a full progress curve in every well for IC₅₀, mechanism, and selectivity studies.
Real-time kinetic or TRF endpoint formats
Optimized substrates and assay-ready kits
Compatible with physiological ATP and cofactors
Designed for: drug discovery, lead optimization, and mechanistic kinase research teams
PhosphoSens-Kinetic assays directly quantify enzyme activity by continuously
monitoring substrate phosphorylation or dephosphorylation in real time,
generating a full progress curve in every well.
PhosphoSens-Red assays use europium-based time-resolved fluorescence detection
to enable robust, plate-based quantification of enzyme activity, making them
well suited for high-throughput screening workflows.
Wild-type and mutant forms of EGFR for assay development, kinase profiling, and mechanistic studies.
Enzymes are supplied active and optimized for PhosphoSens® substrates.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity/Activity: For specific information on a given lot, see related technical data sheet.
Storage Conditions: Store product at â70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
How many assays can I run per 1 mg of Sensor Peptide?
PhosphoSens Sensor Peptides range in molecular weight (MW) based on the size and sequence of the substrate. The average MW or a sensor peptide is 2,150 g/mol. This size Sensor Peptide will be re-suspended in approximately 450 µL of diluent. If running an assay in a 384-well plate, with a final well volume of 20 µL and Sensor Peptide concentration of 10 µM, you can run 2,250 assays!
How do I resuspend my lyophilized sensor peptide?
Reference the "Solubilization" section of the printed Certificate of Analysis (CofA) that is printed and shipped with every order. If you do not see this, please contact us at support@assayquant.com and we will share an electronic copy of your CofA.
What is a reagent pack?
Reagent packs include optimized buffers, cofactors, and detection reagents required to run PhosphoSens® assays. They are used with bulk substrates or custom assay workflows.
Explore data and documents to support your kinase and phosphatase experiments. Download sample
data, protocols and other resources to see how our assays perform and to help you get started in your own lab.
All validation data generated using PhosphoSens® assays under recommended conditions.
AQT0734 - EGFR Assay Validation Report
Each validation report provides experimental conditions and data showing:
Enzyme titration and linearity
Sensor peptide substrate titration and Km determination
Discover how continuous assay formats power deep understanding of kinase function. See how
PhosphoSens® assays guide inhibitor profiling, selectivity assessment, and
mechanistic characterization.