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Kinase Activity Assays

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Continuous Kinase Activity Assays For Real-Time Progress Curves In Every Well

AssayQuant's PhosphoSens detection technology delivers a new standard for measure kinase activity by directly monitoring phosphorylation of a peptide substrate throughout the entire reaction. These assays capture real-time data at the substrate level, offering richer insights into enzyme kinetics, inhibitor interactions, and reaction dynamics—without the limitations of traditional endpoint assays.

Validated Assays For 500+ Protein Kinases

EXPLORE KINASE ACTIVITY ASSAYS CATALOG

Flexible Configurations Built Around Our Substrates For Your Applications

PhosphoSens Substrates

Starting at $2,695

AssayQuant’s patented PhosphoSens® Technology is packed into thoughtfully designed peptide substrates for optimal target specificity. Each PhosphoSens Senor Peptide Substrate can be purchased as lyophilized 1mg aliquots. Each mg is sufficient for 2,000 reactions.

 

Substrate Systems

Starting at $3,095

Designed for higher throughput applications AND ease of use, each Substrate System includes sufficient volumes of substrate, enzyme and reaction reagents to perform 2,000 reactions.

Includes:

  • 1mg PhosphoSens Substrate
  • ATP, EGTA, DTT, Reaction Buffer and Dilution Buffer

Evaluation Kits

Starting at $295

Designed for streamlined technology assessment, each discovery kit includes sufficient volumes of substrate and reaction reagents to perform 200 reactions.

Includes:

  • PhosphoSens Substrate
  • ATP, EGTA, DTT, Reaction Buffer and Dilution Buffer

Kinase Activity Systems

Starting at $3,495

Ready-to-use systems include everything needed for fast, sensitive, and streamlined assay setup. Includes sufficient volumes of substrate, enzyme and reaction reagents to perform 2,000 reactions.

Includes:

  • 1mg PhosphoSens Substrate
  • 10ug Recombinant Kinase
  • ATP, EGTA, DTT, Reaction Buffer and Dilution Buffer
NEW - 900+ Active Protein Kinases

We know that the success of research and drug discovery projects relies on the activity of your target and the ability to quantify that activity.

High-purity, assay-validated enzymes that seamlessly integrate with PhosphoSens assays for improved enzyme and inhibitor activity studies. 

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Features & Benefits

Revolutionizing Kinase Research and Drug Development with Continuous Activity Assays

Technical Advantage

Continuous assays are used to determine binding kinetics and selectivity of your lead compounds, including:

  • Reliable and accurate quantification of compound potency
  • Superior selectivity assessments and insight into compound-dependent kinetics & MOA with kinome profiling at low or high ATP
  • Deep characterization and determination of kinetic parameters for reversible (residence time (τ), IC50 or Ki) or irreversible (kinact, KI, kinact/KI)) inhibitors

Workflow Advantage

PhophoSens assays use a simple protocol that generates a full progress curve in every well.
One-step homogeneous assay. Simply add-and-read.

There are no washing or separation steps and no stop solutions or additional components needed to develop the fluorescence signal.

Continuously measure the catalytic event of phosphorylation in real-time so you can simply set up the number of wells per compound doses you want to study, start reading, and walk away.

Cost Advantage

Measuring the cost of each assay per well is a thing of the past! To determine an inhibitors IC50  with PhosphoSens, there is no need to for extensive protocol modifications or the need to set up time course studies that require repeatedly conducting the multiple step enzyme-coupled reaction. Simply add and read and you will generate dozens of data points in each well, maximizing your reagents and most importantly, your time.

Continuous, Real-Time Monitoring

Captures the entire kinetic profile from start to finish. This approach yields the actual reaction rate, with high accuracy, precision, and confidence.

Direct Measurement of Enzyme Activity

Measures enzyme activity at the substrate level, avoiding the complications of indirect assays that require additional steps.

Physiologically Relevant Conditions

Use biologically relevant peptide substrate sequences in assays that are compatible with low to physiological [mM] concentrations of ATP.

Single-Step, Homogenous Workflow

Achieve fast and reproducible results with a homogenous, single-step workflow without compromising data quality.

INTRODUCTORY OFFER

The Best Solutions Start with a Conversation

Not sure which product or service is the best fit for your project? At AssayQuant, our team of scientific experts can provide the guidance and support you need to achieve your research goals.

SCHEDULE YOUR COMPLIMENTARY CONSULTATION TODAY!
Featured Products

JAK1 Kinase Activity System

$3,495
 
Includes:
  • 1 mg PhosphoSens Sensor Peptide Substrate AQT0663
  • 10 µg Active JAK1 Kinase (438-end, GST Tag)
  • PhosphoSens-Kinetic Reagent Pack
Assay capacity:
Supports approximately 2,000 assays. Actual assay count varies with enzyme concentration and substrate use. Refer to the validation report for detailed guidance.
 
Access JAK1 (JH1-JH2) Assay Validation Report View JAK1 Products Page
How It Works

Discover Your Next Enzyme Activity Assay

PhosphoSens-Kinetic assays leverages AssayQuant’s Proprietary Detection Technology to provide real-time, continuous measurements of kinase activity by directly detecting substrate phosphorylation throughout the kinase reaction.

Sensor Peptide Substrates are developed with our readout molecule, Sulfonamido-oxine (Sox), covalently attached via a cysteine residue (native or introduced) in close proximity to the Serine, Threonine, or Tyrosine phosphorylation site (± 2-5 residues). 

The Sox fluorophore reports peptide phosphorylation by the target kinase via chelation-enhanced fluorescence (ChEF). The fluorescence-sensing mechanism is based on the chelate effect introduced by phosphorylation of the Ser/Thr/Tyr residue proximal to CSox, which enhances magnesium (Mg²⁺) binding affinity compared to the unphosphorylated species, and results in increased fluorescence emission intensity (2-10-fold).

In this way, CSox-modified peptide substrates provide a fluorescent intensity signal that is directly proportional to the phosphorylation level of the substrate, enabling continuous/real-time kinetic measurements of kinase activity in medium to high throughput (96−1536 well plate).

Asset 2-100
STEP 1

Add

Active enzyme is added to reaction mix containing a Sensor Peptide Substrate, catalyzing phosphotransfer from ATP to the substrate in real time.

STEP 2

Read

Add plate to reader and monitor kinase activity throughout the reaction. Signal is directly proportional to the phosphorylation level of the substrate. 

Resources & Protocols

Understand How Our Technology Can Support Your Next Discovery

WEBINAR

Deep Kinetic Characterization: When IC50 Isn't Enough

A continuous assay, like our PhosphoSens assay, that measures the catalytic event of phosphorylation in real time enables determination of kinetic parameters that power your drug discovery research.

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BLOG

Continuous vs. Endpoint Kinase Assays: What You Need to Know

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PROTOCOL

PhosphoSens-Kinetic Kinase Inhibitor IC50 Determination 

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