AQT0658 PhosphoSens® Tyrosine Kinase Substrate
A PhosphoSens® tyrosine kinase substrate validated for 4 kinases (3 wild-type, 1 mutant), including MET, EPHA1, EPHA5. Continuous, direct, real-time activity measurement — no antibodies, no coupling enzymes, no radioactivity.
- Catalog number
- AQT0658
- Enzyme class
- Kinase
- Residue specificity
- Tyrosine
- Validated kinases
- 4
- Assay formats
- Kinetic · 3 Red · 1
- Kinase families
- 2
Kinases validated with AQT0658
Every kinase below has a validated PhosphoSens® assay using AQT0658. Select a target to see its kits, recombinant enzyme, pricing and protocols.
| Kinase | Type | Family | Format with AQT0658 | Link |
|---|---|---|---|---|
| EPHA1 | Wild-type | Eph | Kinetic | View assay → |
| EPHA5 | Wild-type | Eph | Kinetic | View assay → |
| MET | Wild-type | Met | Red | View assay → |
| MET [Y1230H] | Mutant | Met | Kinetic | View assay → |
No kinases match that search.
Bulk substrate
AQT0658 PhosphoSens® Kinase Substrate
Bulk substrate for assay development, screening and high-throughput workflows. Kinase assay kits that include AQT0658 are on each target's assay page.
How PhosphoSens® substrates work
PhosphoSens® substrates are peptides carrying an environment-sensitive fluorophore next to the phospho-acceptor residue. When the kinase transfers a phosphate to the substrate, fluorescence increases, so activity is read continuously and directly in a plate reader.
- Real-time progress curves, not single end-points
- Run at physiologically relevant ATP concentrations
- No antibodies, coupling enzymes, wash steps or radioactivity
- One substrate across 4 kinases simplifies selectivity and panel work
Frequently asked questions
Which kinases is AQT0658 validated for?
AQT0658 is validated for 4 kinases (3 wild-type and 1 mutant), including MET, EPHA1, EPHA5. See the full list above.
Which PhosphoSens assay formats use AQT0658?
AQT0658 is the PhosphoSens-Kinetic substrate for 3 kinases. It is the PhosphoSens-Red substrate for 1 kinase.
What sizes is AQT0658 available in?
1 mg, 5 mg, 10 mg and 20 mg, priced from $2795 (1 mg) to $37285 (20 mg) USD.