Cell Lysate Kinase Assays
Real-Time Kinetic Protein Kinase Activity Assays in Complex Biological Samples – enabling precise quantitation and deeper insight into kinase and inhibitor function in a biologically relevant context.
Quantify Endogenous Kinase Activity and Signaling in Cell Lysates and Tissue Homogenates - No Antibodies, No Washes, Just a Continuous Signal
AssayQuant’s PhosphoSens detection technology sets a new standard for measuring kinase activity by directly monitoring the phosphorylation of highly selective peptide substrates in real time. To support the growing needs of kinase research and drug development, we’ve paired this innovative detection platform with high-throughput peptide synthesis to design sensor peptide substrates with exceptional target selectivity. By capturing continuous data at the substrate level, PhosphoSens assays provide richer insights into enzyme kinetics, inhibitor interactions, and reaction dynamics—without the limitations of traditional endpoint assays.
Validated Assays For ERK1/2, AKT1/2/3, GSK3A/B & many more in development
Flexible Configurations Built Around Our Substrates For Your Applications
Cell Lysate Assay Kit (100 Assays)
AQTxxxx-KL-100
$495
Designed for streamlined technology evaluation, each kit includes sufficient volumes of substrate, reaction reagents, cell extraction reagents and controls to perform 100 reactions at 15 μM substrate in a 384-well plate (25 μL reaction volume).
Includes:
- PhosphoSens Kinase-Selective Lysate Substrate
- ATP, EGTA, DTT, Enzyme Reaction Buffer and Enzyme Dilution Buffer
- PhosphoPreserve Cell Extraction Buffer, Protease Inhibitor and Phosphatase Inhibitor Cocktails
- PhosphoControl Peptide, Recombinant Enzyme, Cell Lysate and Reference Compound
Cell Lysate Assay Kit (1,000 Assays)
AQTxxxx-KL-1000
$3,195
Designed for streamlined technology evaluation, each kit includes sufficient volumes of substrate, reaction reagents and cell extraction reagents to perform 1,000 reactions at 15 μM substrate in a 384-well plate (25 μL reaction volume).
Includes:
- PhosphoSens Kinase-Selective Lysate Substrate
- ATP, EGTA, DTT, Enzyme Reaction Buffer and Enzyme Dilution Buffer
- PhosphoPreserve Cell Extraction Buffer, Protease Inhibitor and Phosphatase Inhibitor Cocktails
Kinase-Selective Lysate Substrate (1mg)
AQTxxxx-L-01
$3,500
AssayQuant's Cell Lysate Kinase Assays combine PhosphoSens Detection Technology with high-throughput peptide synthesis to design sensor peptide substrates that are highly selective for your targets of interest.
Each Kinase-Selective Lysate Substrate can be purchased as lyophilized 1mg aliquots. Each mg is sufficient for 1,500 reactions at 15 μM substrate in a 384-well plate (25 μL reaction volume).
Bulk Pricing available.
Cell Lysate Assay Reagent Pack
AQT-LRP
$600
Designed for end user flexibility, incorporate our optimized reagents into your workflow. These reagent packs include sufficient volumes of reaction reagents and cell extraction reagents to perform 2,000 reactions in a 384-well plate (25 μL reaction volume).
Includes:
- ATP, EGTA, DTT, Enzyme Reaction Buffer and Enzyme Dilution Buffer
- PhosphoPreserve Cell Extraction Buffer, Protease Inhibitor and Phosphatase Inhibitor Cocktails
Cell Lysate Assay Controls
Contact Us For Pricing
Ready to scale up from 100 assays but still want the positive controls we used to develop and validate the assay?
Contact us at hello@assayquant.com and we will work with you to get the right amount of controls for your experiment.
Shop Cell Lysate Kinase Assays By Target
Use the table below to find the Cell Lysate Kinase Assay that matches your target of interest. Each Kinase-Selective Lysate Substrate is identified by its unique AQT code (e.g., AQT1076).
To place an order, simply email us with the desired product format:
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AQTxxxx-KL-100: 100-assay Lysate Kit
-
AQTxxxx-KL-1000: 1,000-assay Lysate Kit
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AQTxxxx-B-01: 1 mg of selective lysate-compatible substrate
Need a quote or help choosing the right configuration?
Click "Request a Quote" below or reach out directly to our team.
| Target Name | HGNC Approved Full Name | Kinase-Selective Lysate Substrate | Sensor Peptide MW (g/mol) |
Assay Validation Report | Target Specific Protocol |
|---|---|---|---|---|---|
| ERK1/2 | mitogen-activated protein kinase (MAPK3/1) | AQT1076 | 2372.95 | LINK > | LINK > |
| AKT1/2/3 | AKT Serine/Threonine Kinase 1/2/3 | AQT0982 | 3204.69 | LINK > | LINK > |
| GSK3A/B | glycogen synthase kinase 3 alpha/beta | AQT1121 | 2005.92 | LINK > | LINK > |
Download Our Comprehensive Cell Lysate Kinase Assay Kit Components Tables
Looking for more detail? Download our complete product list, which includes component tables for each lysate assay.
Revolutionizing Kinase Research and Drug Development with Continuous Activity Detection in Complex Biological Samples
By using unfractionated cell lysates or tissue homogenates, our assays let you measure kinase activity in a native, biologically relevant environment. This enables you to examine kinase complexes from multiple sample types and link target inhibition to broader signaling network status and disease relevance.
Unlike simplified biochemical assays, our format maintains the complexity of the cellular milieu, capturing key factors that affect kinase behavior and inhibitor performance in vivo:
Cellular Components
Native proteins, lipids, nucleic acids, and cofactors that form functional complexes.
Post-Translational Modifications (PTMs)
Natural modifications that regulate kinase activity and interactions.
Signaling Networks
Dynamic feedback and crosstalk, including off-target enzymes that impact selectivity and potency.
Direct, Real-Time Detection of Kinase Activity in Complex Samples
AssayQuant's Cell Lysate Kinase Assays combine PhosphoSens Detection Technology with high-throughput peptide synthesis to design sensor peptide substrates that are highly selective for your targets of interest.
These Kinase-Selective Lysate Substrates are developed with our readout molecule, Sulfonamido-oxine (Sox), covalently attached via a cysteine residue (native or introduced) in close proximity to the Serine, Threonine, or Tyrosine phosphorylation site (± 2-5 residues).
The Sox fluorophore reports peptide phosphorylation by the target kinase via chelation-enhanced fluorescence (ChEF). The fluorescence-sensing mechanism is based on the chelate effect introduced by phosphorylation of the Ser/Thr/Tyr residue proximal to CSox, which enhances magnesium (Mg²⁺) binding affinity compared to the unphosphorylated species, and results in increased fluorescence emission intensity (2-10-fold).
In this way, CSox-modified peptide substrates provide a fluorescent intensity signal that is directly proportional to the phosphorylation level of the substrate, enabling continuous/real-time kinetic measurements of kinase activity in medium to high throughput (96−1536 well plate).
Prepare your Sample
Grow cells (+/- stimulus), aspirate medium and wash, and add PhosphoPreserve Cell Extraction Buffer with Protease and Phosphatase Inhibitors Cocktails to lyse the cells.
Start Kinase Reaction
Sample is added to reaction mix containing Target-Selective Sensor Peptide Substrate, catalyzing phosphotransfer from ATP to the substrate in real time.
Measure Activity
Add plate to reader and monitor kinase activity throughout the reaction. Signal is directly proportional to the phosphorylation level of the substrate.
Selective Kinase Substrate Development
While our current portfolio of biochemical assays are designed for purified kinases and optimized for activity and sensitivity, the running assays in complex samples requires an added layer of selectivity. That’s because lysates contain many active kinases, requiring our new sensors to be engineered to detect the intended target without interference—ensuring accurate, meaningful data in complex sample types. To develop these substrates, we offer Custom Assay Development Services which focuses on a Milestone-based approach.
Milestone 1
- Screened 123 candidate sensor peptides from derived from native substrate sequences.
- Identified AQT0491, with high activity but moderate selectivity.
Milestone 2
- Screened 259 sequences for ERK1/2 activity and selectivity.
- Selected optimized single amino acid substitutions that enhance activity and specificity.
Milestone 3
- Combined substitutions to generate 194 peptide variants.
- Top-performing peptide, AQT1076, showed 10x better selectivity and 1.5x higher ERK2 activity than AQT0491.
The Best Solutions Start with a Conversation
Not sure which product or service is the best fit for your project? At AssayQuant, our team of scientific experts can provide the guidance and support you need to achieve your research goals.
Additional Targets In Development - Subscribe for updates!
We are actively developing Cell Lysate Kinase Assays for the following targets: AKT, MEK, PIM, JNK, P38, GSK3, CDK and more!
Understand How Our Technology Can Support Your Next Discovery
A Continuous Kinetic Assay to Quantitate Specific Protein Kinase Activity in Unfractionated Cell Lysates
This poster will fully describe the methods and results pertaining to ERK-selective sensor peptide development and validation and the ability to extend to other kinases of interest.
Continuous vs. Endpoint Kinase Assays: What You Need to Know
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